DNS法测定还原糖含量时最适波长的确定_王俊丽.pdfVIP

河南农业科学 DNS 王俊丽, 聂国兴, 李素贞, 谢艳敏, 曹香林 ( , 453002) : 探讨DN S 法测定还原糖含量时的最适波长以DN S 为显色剂, 用分光光度法分析测定了 木糖和葡萄糖显色 的最大吸收波长和最适测定波长结果表明, 木糖和葡萄糖与D N S 显色剂反 应后, 均在490 nm 处有最大吸收峰标准曲线糖含量范围为0 ~ 40 mg / L 时, O D 值在520nm 处 的R 2 最接近于1, 精确度和回收率均较高提示DN S 法测定还原糖含量时, 520 nm 为最适测定 波长 : 还原糖; 木糖; 葡萄糖; DN S 法; 最适波长 : Q503 : A : 1004 - 3268( 2010) 04- 0115- 04 Opt im a Wave ength f or Det erm ining the Cont ent of Reducing Sugar by DN S M et hod WA N G J un- i, N IE Guo-xing, L I Su- zhen , XIE Y an- m in , CA O X iang- in ( Co ege of L if e Sciences, Henan No rma U niver sity, Xinx iang 453002 , China) Abstract: In t his study , t he OD va ues of x y ose and g ucose under diff erent w ave engt h w ere de- t ermined. T he r esu t s show ed t hat t he D N S r eaction so ut ion of xy ose and g ucose hav e t he same maxim um absorpt ion w ave eng t h ( 490 nm ) and t he sam e opt ima det ermining w ave engt h ( 520 nm) . When determ ine t he content of x y ose and g uco se under t he w ave eng th o f 520 nm, the precision and reco ectio n rate are comparative y high and the ine rang e o f reducing sug ar is 0~ 40m g/ L . Key words: Reducing sug ar; Xy ose; G ucose; DN S met hod ; Opt ima w ave eng th : 2009- 11- 15 : ( 092102210 127) ; : ( 1971- ) , , , , , * : ( 1971- ) , , , , , [ 9] P eng D, Hu S, H ua Y , et al . Co mpar ison o f a new g o d 2 86- 293. immunochromat og raphic assay fo r the detect ion o f an- [ 12] Zhang G P, W ang X N , Y ang J F , et al . Dev e opment tibodies against av ian inf uenza viru s w ith hemagg uti- of an immunochro matog raphic at era f ow test str ip nat