GC_MS方法测定人血浆中龙脑药物含量的方法学研究.pdfVIP

- 43 - 1 300070 2 300193 3 300193 4 300072 GC / MS HP-WAX 30 m × 0.25 mm × 0.25 μm 150 5.5 min 40 /min 190 1 min 5 ∶1 （SIM ） m/z 95 m/z 128 4.56 min 5.31 min 5 ~ 1 000 ng/mL （r = 0.999 3 ） 3 80 % R969.1 A 1674–6376200901–0043–05 Establishment and validation of GC/MS method for the determination of borneol in human plasma 1 2 3 2 4 2 LI Hua-long , LU Rong , ZHAO Li-xia , PANG Si-lin ， , SI Duan-yun 1 Tianjin Institute for Drug Control, Tianjin 300070, China 2 Tianjin State Key Laboratory of Pharmacokinetics and Pharmacodynamics, Tianjin Institute of Pharmaceutical Research, Tianjin 300193, China 3 Tianjin Institute of Pharmaceutical Responsible Company Ltd, Tianjin 300193, China 4 Department of Pharmaceutical Engineering, School of Chemical Engineering and Technology, Tianjin, University, Tianjin 300072, China Abstract Objective: A sensitive and selective GC/MS method has been developed to determine the level of borneol in human plasma after po ShuXinPing Capsules. Methods: The quantitative analysis were carried out by using a HP-WAX silica capillary column 30 m × 0.25 mm × 0.25 μm. The column was maintained at 150 ℃ for 5.5 min, then the temperature was increased to 190 ℃ which was kept for 1 min at the speed of 40 ℃ /min. Helium was applied as carrier gas. The splitting-ratio was 5 ∶1. Sele