有机酸胁迫下厌氧污泥产氢效果.pdfVIP

微生物学通报 APR 20, 2010, 37(4): 529−533 Microbiology China © 2010 by Institute of Microbiology, CAS tongbao@ 研究报告 有机酸胁迫下厌氧污泥产氢效果 1,2 1,2 1,2* 2,3 黄振兴 严群 阮文权 余晓斌 (1. 214122) (2. 214122) (3. 214122) : 4 , 摘 要 乙酸、丙酸、乳酸及丁酸是厌氧发酵产氢过程中 种主要的液相末端发酵产物 其积累对 产氢过程有一定的抑制作用。实验利用多种有机酸胁迫提高污泥的酸耐受能力, 并以污泥中脱氢酶 活性为生化指标, 对不同浓度酸胁迫下厌氧污泥活性变化进行了研究。通过对胁迫后污泥产氢量及 末端产物进行对比。结果表明, 酸胁迫后污泥产氢量有一定增加, 其中乙酸和丁酸胁迫效果最好, 较 对照组提高了近一倍; 末端产物分析研究表明, 不同的有机酸胁迫后, 其产量在发酵过程中都有一 定的增加, 而乙酸含量在酸胁迫后都有不同程度的提高。 关键词: 厌氧发酵, 胁迫, 有机酸, 末端产物, 酸应激 Effect of Organic Acids Stress on Biohydrogen Production by Anaerobic Granular Sludge HUANG Zhen-Xing1,2 YAN Qun1,2 RUAN Wen-Quan1,2* YU Xiao-Bin2,3 (1. School of Environmental and Civil Engineering, Jiangnan University, Wuxi, Jiangsu 214122, China) (2. Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, Jiangsu 214122, China) (3. School of Biotechnology, Jiangnan University, Wuxi, Jiangsu 214122, China) Abstract: The major organic acids produced in the fermentative hydrogen production by mixed cultures were acetic acid, propionic acid, lactate acid and butyric acid, etc. which had negative effect on hydrogen pro