大鼠黑质多巴胺能神经元二型性.pdfVIP

生理学报 Acta Physiologica Sinica, December 25, 2007, 59 (6): 753-758 753 研究论文 大鼠黑质多巴胺能神经元的二型性 马元怡，孔淑珍，杨黎江，孟金兰，吕乐春，何敏* 昆明医学院生理教研室，昆明 650031 摘 要：成年哺乳动物的某些脑区存在性别差异，即二型性，但中脑黑质是否存在性分化目前不清楚。本文旨在探讨成 年大鼠中脑黑质是否存在二型性。将60 只成年大鼠分成5 组：(1) 正常雌鼠对照组；(2) 正常雄鼠对照组；(3) 去卵巢组；(4) 去睾丸组；(5)去卵巢后回补雌激素组，该组大鼠在去卵巢后的第7 天开始连续3 d 给予生理剂量的雌激素回补。所有大鼠 在右侧黑质埋置记录电极，在清醒和安静的生理状态下连续14 d 记录黑质的P50 听觉诱发电位(P50) ，之后作黑质酪氨酸 羟化酶(tyrosine hydroxylase, TH)免疫组织化学染色，检查TH 阳性(TH+ )细胞数量和形态变化。结果表明，正常成年雄鼠 黑质的TH+ 细胞数量较雌鼠少22.47% (P0.05) ，P50 的T/C 值也低34.72% (P0.01) ，提示正常成年大鼠黑质在结构和功能 上存在二型性。与正常雄鼠相比，去睾丸大鼠黑质的TH + 细胞数量、形态和P50 的T/C 值无显著性变化(P 0.05) 。与正 常雌鼠相比，去卵巢大鼠黑质TH+ 细胞数量减少28.09% (P0.01) ，P50 的T/C 值降低30.85% (P0.01) 。在大鼠去卵巢后 的短时间内给予3 d 生理剂量的雌激素，15~20 d 后可观察到其黑质TH+ 细胞数量、形态和P50 的T/C 值基本恢复到去卵 巢前水平。结果提示，大鼠中脑黑质的多巴胺能神经元在数量、结构和功能活动上存在性别差异；内源性雌激素在维持 黑质多巴胺系统完整性及调节其功能活动中起重要作用。 关键词 ：脑的二型性；雌激素；黑质；多巴胺神经元；感觉门控；P 5 0 听觉诱发电位；酪氨酸羟化酶 中图分类号：R 3 3 8 Sexual dimorphisms of dopaminergic neurons in rat substantia nigra MA Yuan-Yi, KONG Shu-Zhen, YANG Li-Jiang, MENG Jin-Lan, LV Le-Chun, HE Min* Department of Physiology, Kunming Medical College, Kunming 650031, China Abstract: There are sex differences in some brain areas in mammalians. Parkinson’s disease (PD), caused by the mesencephalic substantia nigra (SN) dopaminergic neuronal loss, displays sexual difference, i.e., the incidence is higher and the symptoms are more intense in males than that in females. However, it has not been known whether sexual dimorphisms exist in the SN. Sixty adult Sprague- Dawley rats were randomly divided into 5 groups: (1) Female intact group (F-INT group); (2) Male intact group (M-INT group); (3) Ovariectomized group (OVX group); (4) Castration group (CAST group); (5) Ovariectomized + estrogen-replaced group (OVX-E2 group): The rats received sequentially physiological dose of estrogen for 3 d from the 7th day after ovariectomization. P50 auditory evoked potential (P50)