生物光学在蛋白质组学中应用.pdfVIP

CHAPTER 15 BIOPHOTONICS APPLIED TO PROTEOMICS MICHEL FAUPEL, DÉBORA BONENFANT, PATRICK SCHINDLER, ERIC BERTRAND, DIETER MUELLER, MARKUS STOECKLI, FRANCIS BITSCH, TATIANA ROHNER, DIETER STAAB, and JAN VAN OOSTRUM Novartis Institutes for Biomedical Research, Basel Table of Contents Abstract 323 1. Introduction 323 2. Two-Dimensional Electrophoresis 324 3. Proteomics Analysis by Mass Sectrometry 325 4. Mass Spectrometry Imaging 329 4.1. Principles and Methods 330 4.2. Compound Imaging 331 4.3. Peptide Imaging 332 4.4. Protein Imaging 333 5. Conclusions 336 References 337 Abstract: Since the completion of the human genome sequencing, our understanding of gene and protein function and their involvement in physiopathological states has increased dramati- cally, partly due to technological developments in photonics. Photonics is a very active area where new developments occur on a weekly basis, while established tools are adapted to fulfill the needs of other disciplines like genomics and proteomics. Biophotonics emerged at the interface of photonics and biology as a very straightforward and efficient approach to observe and manipulate living systems. In this chapter, we review the current applications of photonics and imaging to proteomics from 2D gels analysis to molecular imaging. 1. INTRODUCTION The complete set of genes of an organism composes its genome, while the complete set of proteins of a cell or tissue constitutes its proteome. Whereas the genome of cells remains stable through their lifetime, the cellular proteome changes in response to the 323 E. Bertrand and M. Faupel (eds.), Sub