klotho蛋白免疫组化定位.pdfVIP

CELL STRUCTURE AND FUNCTION 29: 91–99 (2004) © 2004 by Japan Society for Cell Biology Immunohistochemical Localization of Klotho Protein in Brain, Kidney, and Reproductive Organs of Mice 1 2 1 2 1 Shun-Ai Li , Masami Watanabe , Hiroshi Yamada , Atsushi Nagai , Masahiro Kinuta , and Kohji Takei1 1 Department of Neuroscience, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan and 2Department of Urology, Okayama University Graduate School of Medicine and Dentistry, 2-5-1 Shikata-cho, Okayama 700-8558, Japan ABSTRACT. Klotho mutant mouse (kl-/-), a mouse model for human aging, exhibits various phenotypes in a wide range of organs including arteriosclerosis, neural degeneration, skin and gonadal atrophy, pulmonary emphy- sema, calcification of soft tissues, and cognition impairment. Klotho mRNA, however, is expressed only in brain, kidney, reproductive organs, pituitary gland, and parathyroid gland. Therefore it remains to be elucidated how lack of Klotho protein in these limited organs leads to the variety of phenotypes. To shed light on mechanisms by which Klotho protein acts on distant targets, we examined localization of Klotho protein in brain, kidney, and reproductive organs, and analyzed brain and kidney in kl-/- mice searching for changes in target regions in these organs. In brain, Klotho proteins were localized at choroid plexus, where the proteins were dominantly localized at the apical plasma membrane of ependymal cells. In kl-/- brain, reduction of synap