Amino Acid Composition and Functional Properties of Giant Red Sea Cucumber (Parasfichopus californicus) Collagen Hydrolysates.pdfVIP

Amino Acid Composition and Functional Properties of Giant Red Sea Cucumber (Parasfichopus californicus) Collagen Hydrolysates.pdf

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Amino Acid Composition and Functional Properties of Giant Red Sea Cucumber (Parasfichopus californicus) Collagen Hydrolysates.pdf

OceanUniv.China OceanicandCoastalSeaResearch1 D0I10.1007/s11802.0l1一l7874 ISSN 1672—5182,20I110 1 :80—84 http://www.ORe.edu.cn/xbywb/ E—mail.xbywb@OblC.edu.cn AminoAcidCompositionandFunctionalPropertiesofGiant RedSeaCucumber Parastichopuscalifomicus CollagenHydrolysates LIU Zunying¨SU Yicheng ,andZENGM ingyong , , 1、College吖FoodScienceandEngineering,OceanUniversityDfChinaQingdao266003PRChina 2 SeaJoodResearchandEducationCenter,OregonStateUniversity,2001MarineDrive,Room253,Astoria,OR97103U S.4 , ReceivedMay10,2010;revisedJuly15,201O:acceptedJuly19,2010 @ OceanUniversityofChina.SciencePressandSpring—VerlagBerlinHeidelberg2010 Abstract Giantredseacucumber Parastichopuscalfiornicus isanunder—utilizedspeciesduetoitshightendencytoautolysis , Iheaim ofthisstudywastoevaluatethefunctionalpropertiesofcollagenhydrolysatesrfointhisspecies . Thedegreeofhydrolysis DH ,aminoacidcomposition,SDS— ofam stability FS ofhydrolysateswereinvestigated.TheeffectsofpHontheEAI,ESIFEandFSofhydrolysateswerea1soinves. tigated.Theresultslndicatedthatthe∥andalchainsofthecollagenwcrceffectivelyhydrolyzedbytrypsinat50℃ withanEn. zyme/Substrate E/S rationof1:20 w:w .TheDHofcollagenwasupto17,3%after3hhydrolysiswithtrypsinThehydrolysates hadamolecularweightdistributionof1.1—17kDa,andwereabundantinglycine Gly ,proline Pro ,glutamicacid Glu .alanine Ala andhydroxyproline Hyp residues.Thehydrolysateswerefractionatedintothreerfactions 3kDa3-10kDa,and 10kDa1 andtherfactionof3-10kDaexhibitedahigherEA1valuethantherfactionof 10kDa P 0 . 05 .Therfactionof 10kDahadhigher FEandFSvaluesthanotherrfactions 户 0.05 .ThepHhadanimportanteffectoiltheEAI,ESI,FEandFS.Alltherfactionsshowed undesirableemulsionandformingpropertiesatpH4.0 . UnderpH7,0andpH 10.0.the3—10kDarfactionshowedhigherEAIvalue andtherfactionof 10kDashowedhigherFEvalue,respectively . Theyarehopedtobeutilizedasfunctionalingredientsinfoodand nutraceutica1jndustries. Keywords giantredseacucumber;collagen;hyrdolysates;ufnctionalproperties enz

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