水稻蛋白质近红外定量模型的创建和在育种中的应用.pdfVIP

2006,39(4):836-841 Scientia Agricultura Sinica 1 2 ( 100094 100094 3 100094) Establishment of Math Models of NIRS Analysis for Protein Contents in Seed and It′s Application in Rice Breeding 1 1 2 3 1 LI Jun-xia , ZHANG Hong-liang , YAN Yan-lu , MIN Shun-geng , LI Zi-chao 1 Key Laboratory of Crop Genomics and Genetic Improvement, Ministry of Agriculture / Key Laboratory of Crop Genetic Improvement, China Agricultural University, Beijing 100094 ; 2 The College of Information, China Agricultural University, Beijing 100094; 3 The College of Science, China Agricultural University, Beijing 100094 Abstract: ObjectiveTo analyze the genetic variability of protein content in rice hybrid progenies, the feasibility of building calibration model to estimate crude protein content in intact rice kernel by near-infrared reflectance spectroscopy (NIRS) was studied. It is beneficial to provide the parameter and improve nutritional quality in grain breeding program.MethodUsing 191 rice samples with a broad range of crude protein content, 5.90%14.50%, as materials, the chemometrical method of partial least square regression was used to establish the calibration model. Result The optimal model was developed by the spectral data pretreatment of the first derivative + vector normalization in 11 998.9 cm-1–5 449.8 cm-1 and 4 601.3 cm-14 246.5 cm-1, by analyzing different spectral data pretreatment and light frequency ranges. This model’s determination coefficient and relative standard deviation (RSD)