川产桑叶“经霜为上”合理性的LCMS法验证.pdfVIP

MEDICINEANDMATERIAMEDICARESEARCH20lIVOL．22NO．1I 时珍国医围药2011年第22卷第11期LISHIZHEN 川产桑叶“经霜为上合理性的LC—MS法验证 游元元1．万德光2。 Luna toni×4．6 叶进行品质比较。色谱柱为PhenomenexCIg(2)(250mm。5“m)．流动相为甲醇一0．5％磷酸梯度洗脱， 流速为1．0 ml·min～，检测波长320nlll。质谱采用ESI负离子电离方式。结果桑叶的总峰面积、绿原酸及4种黄酮类 物质的峰面积在ll或12月份达最大值。结论川产桑叶“经霜为上”是合理的。 关键词：桑叶；采收期；经霜为上；液一质联用 DOI标识：doi：10．3969／j．issn．1008-0805．2011．11．0ll 中图分类号：R282．71 文献标识码：A 文章编号：1008-0805(2012)1l-2596-02 Test ofFrosted LeavesProducedfromSichuan Better Validity Mulberry Having Quality LC—MS by YOU Yuan-yuan‘，WANDe-guang‘。 Medical (1．PharmacologicalCollege，ChengduCollege，Chengdu College， of 610075，China) ChengduUniversityTCM，Chengdu Tovalidatethefrosted leaves fromSichuan better LC—MS．Methods Abstract：Objective mulberryproduced having qualityby wag 4 from were in LC—MS method leaves onmulberries toDecember analytical established，thegathered August comparedquali— a Lum lain elutionofmethanoland was on Phenomenex ty．HPLC x4．6mm，51．Lm)withgradient performed C18(2)column(250 mass Was acidattherateof1．0ml·rain-1．Thedetection Wassetat320nm．The 0．5％phosphoric wavelength spectmmetryper- formedonESI．ResultsAreaoftotal acid four of leavesreached peaks，chlomgenicpeal【andquercitinglycosidespeaksmulberry themaximuminNovemberorDecember．Conclusio