固定化酶地的研究方法概述.pdfVIP

2011 年第11 期 中 国 酿 造 总第236 期 · · 专论与综述 5 固定化酶的研究方法概述 1 1 1 1 2 1 李小冬，吴 嘉，贾东晨，万永虎，乔 昕，乔 敏* （1.云南大学云南省生物资源保护与利用重点实验室-省部共建国家重点实验室培育基地，云南昆明 650091; 2. 中国储备粮管理总公司云南分公司质检中心，云南昆明 650091） 摘 要：固定化酶在现代的工业生产中扮演着举足轻重的作用，从固定化酶的发展过程着手，重点描述了传统的固定化酶的制备方 法，组合固定化及交联酶晶体（CLECs），交联酶聚集体（CLEAs）等。并对这些方法的研究手段，应用前景进行了介绍。 关 键 词：固定化酶；组合固定化；交联酶晶体；交联酶聚集体 中图分类号：Q55 文献标识码：A 文章编号：0254-5071 （2011）11-0005-05 Review of research methods of enzymes immobilization 1 1 1 1 2 1 LI Xiaodong , WU Jia , JIA Dongchen , WAN Yonghu , QIAO Xin , QIAO Min * ( 1.Laboratory for Conservation and Utilization of Bio-Resource, Yunnan University, Kunming 650091, China; 2.Quality Insp ection Center, Yunnan Branch of China Grain Reserves Corp oration, Kunming 650091, China) Abstract ：Immobilization of enzymes are currently considered as a more useful means in industrial catalysts, the transition in development of immo- bilized enzymes and preparation of enzymes which including fundamental immobilization techniques, combinatorial approaches, cross-linked enzyme aggregations(CLEAs), cross-linked enzyme crystals(CLECs) and so on were reviewed. Research means for these methods and their prospect in indus- trial applications were