酸奶中嗜热乳酸链球菌的分离鉴定和益生特性研究.pdfVIP

安徽农业科学,Journal of Anhui Agri .Sci .2008 ,36(7) :2615 - 2616 责任编辑 陈玉敏 责任校对 马君叶 酸奶中嗜热乳酸链球菌的分离鉴定及益生特性研究 * 李公美, 夏娌君, 单晓枫, 张洪波,钱爱东 ( 吉林农业大学动物科技学院, 吉林长春 130118) 摘要 [ 目的] 探讨酸奶 中嗜热乳酸链球菌的益生特性。[ 方法] 利用MRS 琼脂培养基从酸奶 中分 离嗜热乳酸链球菌, 通过糖发酵试验对 其进行生化鉴定。研 究嗜热乳酸链球菌的抑菌特性和胆盐耐受性, 并分析不 同pH 值对嗜 热乳酸链球菌的的影响。[ 结果] 镜检结果表 明该菌为革兰氏阳性, 经生化鉴定可确定该菌为嗜热乳酸链球菌。嗜热乳酸链 球菌对人体肠道 内的常见病 原菌有抑制作 用, 尤其是对 大肠杆菌和金黄 色葡萄球菌的抑制作 用明显。嗜热乳酸链球菌可以耐受人体肠道的胆盐环境, 并在其特定部位生存 。嗜热乳酸链球菌 可耐受强酸, 能在 胃酸 中存活, 在近 中性pH 值环境 中生长较好 。[ 结论] 嗜热乳酸链球 菌可在人体肠道 内发挥益生作 用, 保护机体免受 病原菌的侵 害。 关键词 嗜热乳酸链球菌; 分离与鉴定; 益生特性; 糖发酵 中图分类号 S879 .1 文献标识码 A 文章编号 0517 - 6611(2008) 07 - 02615 - 02 Study on the Isol ation and Identification of Strept ococcus t her mophilus fromAcidophilus Milk and Its Probiotic Characteristics LI Gong- mei et al ( Subj ect of Preventive Veterinary Medicine , Jilin Agricultural University , Changchun ,Jilin 130118) Abstract [ Obj ective] The ai m of the research was to discuss the probiotic characteristics of Strept ococcus ther mophilus in acidophil us milk . [ Method] S . ther mophilus was isolatedfromaci dophilus milk by using MRS agar mediumto make biochemical i dentification by sugar fer mentationtest . The antibac- terial characteristics and bile salt tolerance of S . ther mophil us were studied and the effects of different pHval ue on S . ther mophil us were analyzed . [ Re- sult] The results of microscope detection showed that this bacteri umwas Grampositive and it was confirmed as S . ther mophi lus through biochemical iden- tification . S . ther mophilus had inhibitory effects on co mmon pathogens in human intesti ne , especially its inhi bitory effects on Escherichia coli and Staphy- lococcus aureus were obvious . S . ther mophilus was tolerant to the bile salt environment in human i ntestine and survived in its specific site . S