复合型微生物絮凝剂产生菌的培养和对炼化废水处理的研究.pdfVIP

　环境污染与防治 　第 33 卷 　第 3 期 　2011 年 3 月 复合型微生物絮凝剂产生菌的培养及对 炼化废水处理的研究 李 　政 　吴伟林 　张云波 　刘其友 　赵东风# ( ( ) ) 中国石油大学 华东 化学化工学院 ,山东 　青岛 266555 　　摘要 　从炼油厂活性污泥中筛选出 5 株微生物絮凝剂产生菌菌株 ,依次编号为SHD1至SHD5 。将任意 2 株复合培养后 ,发 现SHD1和SHD5构建的复合菌对炼化废水的絮凝效果最好 。通过单因子实验确定复合菌的最优培养条件 :培养时间为 48 h ,初始 p H 为 7 ,摇床转速为 160 r/ min 、碳源为葡萄糖 、氮源为蛋白胨 + (N H4 ) 2 SO4 (质量比为 1 ∶1) 。在最优培养条件下 ,复合菌所产复 合型微生物絮凝剂对炼化废水中浊度 、石油类物质和 COD 的去除率分别达到 93 . 45 % 、53 . 42 %和 20 . 98 % ,絮凝效果优于无机絮凝 剂 ,具有广泛的应用前景 。 　　关键词 　复合 　微生物絮凝剂产生菌 　培养条件 　炼化废水 Cultivation of compound bioflocculantproducing bacterium and its application in ref inery wastewater treatment 　L I Z heng , W U Wei l i n , Z HA N G Yunbo , L I U Qiy ou , Z HA O D ongf eng . ( Col leg e of Chem is t ry an d Chem ical Eng i ( ) ) nee ri ng , Chi na Uni ve rs i ty of Pet roleum E as t Chi na , Qi ng d ao S han dong 266555 Abstract : 　Five bioflocculantp roducing st rain s were screened f rom t he activat ed sludge of refining and chemical p lant ; t he five st rain s were named SHD1 to SHD5 in due or der . Aft er two arbit rary st rain s were mixed and cultivat ed , it wa s found t hat t he compound bioflocculantp roducing st rain s SHD1 and SHD5 had t he best t reat ment effect for refinery wa st ewat er . The op timum cult ure condition s of t he select ed st rain s were ascert ained t hrough single factor exp eriment s. The result s showed t hat t he op timum condition s were cult ure p erio d 48 hour s , initial p H 7 , rot ation sp eed 160 r/ min , gluco se a s carbon source , p ep tone + ammonium sulfat e (ma ss ratio 1 ∶1) a s nit ro gen source . U nder