兔成纤维细胞生长因子5(FGF5)基因SNP及其和产毛量的相关分析.pdfVIP

兔成纤维细胞生长因子 5 （FGF5 ）基因 SNP 及其与产毛量的相关分析 李春笑，蒋美山，陈仕毅，赖松家 四川农业大学动物科技学院，雅安 625014 摘要：通过PCR 产物直接测序的方法，对荥经长毛兔、天府黑兔以及加利福尼亚兔的 FGF5 基因外显子 1 和外显子 3 进行单核苷酸多态性分析。在外显子 1 的 217 位(位点 A)检测到由 TCT 三碱基插入引起的移码 突变，在外显子 3 的 59 位(位点 B)和 3 位(位点 C)分别发生了错义突变由 T→C 和同义突变由 T→C 。通过 计算发现各位点不同的基因型和等位基因频率在 3 个兔品种中存在较大的差异，位点 A 、B 在长毛兔和肉 兔中均有各自的优势基因型和等位基因。各位点基因型与产毛量的最小二乘分析表明，位点 A 各基因型的 个体在产毛量上差异不显著(P ＞0.05)，位点B 各基因型个体产毛量的差异极显著(P ＜0.01)，位点 C 各基因 型个体产毛量的差异显著(P ＜0.05) 。初步推断 FGF5 基因可能是影响长毛兔产毛量潜在的主效基因或者与 主效基因连锁，可作为长毛兔产毛性状连锁分析的候选遗传标记。 关键词：兔；FGF5 基因；单核苷酸多态性；产毛量 Correlation analysis between single nucleotide polymorphism of FGF5 gene and wool yield in rabbits LI Chun-Xiao, JIANG Mei-Shan, CHEN Shi-Yi, LAI Song-Jia College of Animal Science and Technology, Sichuan Agricultural University, Yaan 625014, China Abstract: Single nucleotide polymorphism in exon 1 and 3 of fibroblast growth factor (FGF5) gene was studied by DNA sequencing in Yingjing angora rabbit, Tianfu black rabbit and California rabbit. A frameshift mutation (TCT insert) at base position 217 (site A) of exon 1 and a T/C missense mutation at base position 59 (site B) of exon 3 were found in Yingjing angora rabbit with a high frequency; a T/C same-sense mutation at base position 3 (site C) of exon 3 were found with similar frequency in three rabbit breeds. Least square analysis showed that different genotypes had no significant association with wool yield in site A , and had high significant association with wool yield in site B (P ＜0.01) and significant association with wool yield in site C (P ＜0.05). It was concluded from the results that FGF5 gene could be the potential major gene affecting wool yield or link with the major gene, and polymorphic loci B and C may be used as molecular markers for improving wool yield in angora rabbits. Keywords: rabbit; FGF5 gene; SNP; wool yield 收稿日期：2007-11-07; 修回日期：2008-01-1