猪IGF2基因的遗传多态性和其遗传效应分析.pdfVIP

HEREDITAS (Beijing) 2008 2 , 30(2): 179―184 ISSN 0253-9772 研究报告 DOI: 10.3724/SP.J.1005.2008.00179 IGF2 薛慧良, 徐来祥 , 273165 : 采用PCR-SSCP 方法检测胰岛素样生长因子2 (insulin-like growth factor 2, IGF2)基因外显子7, 8, 9 的多 态性, 并分析其对初生重、断奶重、6 月龄重和 6 月龄背膘厚的遗传效应。根据猪 IGF2 基因的 DNA 序列 (AY044828)设计 3 对引物, 结果在 Ex8 引物对扩增的片段上发现了多态性, 并对纯合子进行测序, 发现 exon8 的53 位存在C→T 转换, 且检测到3 种基因型(AA 、AB 、BB) 。统计结果表明, 3 种基因型在各品种中的分布不 一致, 长白猪与大白猪比较, 莱芜猪与大薄莲猪比较, 沂蒙黑猪和里岔黑猪比较差异不显著(P 0.05); 其他猪 种间基因型分布的差异均显著(P 0.01) 。固定效应模型分析结果表明, 初生重和6 月龄背膘厚基因型间差异显 著(P 0.05), 而断奶重和6 月龄重基因型间差异不显著(P 0.05) 。最小二乘分析结果表明, BB 基因型个体同 AA 和AB 基因型个体比较初生重的差异显著(P 0.05), 3 种基因型在初生重的大小排列顺序为AB AA BB ; AA 基因型个体同AB 和BB 基因型个体比较6 月龄背膘厚的差异显著(P 0.05), 3 种基因型在6 月龄背膘厚的大小 排列顺序为BB AB AA 。因此, 推测IGF2 基因对个体的初生重和胴体瘦肉率存在一定的影响, 将IGF2 基因 应用于猪育种过程中的标记辅助选择可以加快猪的育种进程。 : 猪; IGF2 基因; 遗传多态性; 遗传效应 Genetic polymorphisms and genetic effects of IGF2 gene in pigs XUE Hui-Liang, XU Lai-Xiang College of Life Science, Qufu Normal University, Qufu, Shandong 273165, China Abstract: Single nucleotide polymorphisms of the exon7, 8, 9 of the porcine IGF2 gene were tested using PCR-SSCP. Ge- netic effects of the IGF2 gene on birth weight, weaning weight, six-month weight and six-month backfat thickness were analyzed. On the basis of the published DNA sequence (AY044828) of the porcine IGF2 gene, three pairs of primers were designed, and one polymorphism, three genotypes were found in the PCR product amplified with Ex8 primers. C→T transi- tion at 53 site of exon8 was detected by sequencing the homozygotes. The results showed that: firstly, the genotype distribu- tion was not consistent among the different pig breeds. N