大肠癌干细胞基因Bmi-1表达和大肠肿瘤组织病理特征的关系.docVIP

大肠癌干细胞基因Bmi-1表达与大肠肿瘤组织病理特征的关系 黄? 喆 辽宁省肿瘤医院大肠外科， 辽宁省沈阳市? 110042 Correlation between Bmi-1 and clinicopathological features of colorectal cancer Huang Zhe Department of Colorectal Surgery, Liaoning Cancer Hospital Institute, Shenyang 110042, Liaoning Province, China 摘要? 背景：大肠癌干细胞基因Bmi-1 (B-cell-specific moloney murineleukemia virus insertion site)在调节人端粒反转录酶的转录状态和活性情况，对细胞的衰老、癌变具有重要意义。 目的：观察大肠癌干细胞基因Bmi-1的表达及其与肿瘤组织病理学变化之间的关系。 方法：采用实时荧光定量PCR技术对50例大肠癌患者肿瘤组织中Bmi-1 mRNA的表达情况进行检测，采用苏木精-伊红染色和免疫组织化学染色染色SP法测定患者肿瘤组织中Bmi-1蛋白的表达。 结果与结论：大肠癌患者的性别、年龄、肿瘤直径对肿瘤组织中大肠癌干细胞Bmi-1 mRNA的表达差异无显著性意义。在不同性别、年龄、肿瘤直径和Duke分期的大肠癌患者肿瘤组织中大肠癌干细胞Bmi-1蛋白表达差异无显著性意义。肿瘤淋巴结转移和浸润深度超过浆膜层的大肠癌患者Bmi-1 mRNA和蛋白的表达明显高于无转移和浸润深度较低的患者(P? 0.05)。大肠癌Duke分期较高的患者体内大肠癌干细胞Bmi-1 mRNA的表达也较高(P? 0.05)。结果证实，大肠癌干细胞基因Bmi-1的表达与大肠癌的转移、浸润及Duke分期的病理特征密切相关。 中国组织工程研究杂志出版内容重点：干细胞；骨髓干细胞；造血干细胞；脂肪干细胞；肿瘤干细胞；胚胎干细胞；脐带脐血干细胞；干细胞诱导；干细胞分化；组织工程 全文链接： 关键词?：?干细胞；?肿瘤干细胞；?大肠癌；?基因；?Bmi-1；?转移；?浸润；?Duke分期；?分化； ?? Abstract： BACKGROUND:?Bmi-1 (B-cell-specific moloney murineleukemia virus insertion site), a colorectal cancer stem cell gene is of great significance in the regulation of telomere reverse transcriptase activity and transcriptional status as well as cell aging and carcinogenesis. OBJECTIVE:?To investigate the correlation between Bmi-1 expression and clinicopathological changes of colorectal cancer. METHODS:?The expression of Bmi-1 mRNA in carcinoma cells was detected in 50 cases of colorectal cancer using quantitative real-time PCR. The expression of Bmi-1 protein in cancer tissue was detected by hematoxylin-eosin staining and immunohistochemical staining SP method. RESULTS AND CONCLUSION:?Sex, age, and tumor size showed no different effects on the expression of Bmi-1 mRNA. The expression of Bmi-1 protein showed no significant difference in patients who were different in sex, age, tumor size and Duke staging of tumor. The expressions of Bmi-1 mRNA and protein in patients with lymph node metastasis and depth of invasion deeper than the seros