硫酸铍致小鼠肝毒性实验研究.pdfVIP

第十一届中南地区实验动物科技交流会论文集 硫酸铍致小鼠肝毒性的实验研究 1 1 2* 骆金俊 廖永红 1 张朝晖 吴端生 （1.南华大学环境医学与放射卫生研究所；2. 南华大学实验动物学部. 湖南衡阳,421001 ） 【摘要】目的 研究腹腔注射硫酸铍对小鼠的肝脏毒性作用，探讨硫酸铍致肝脏毒性的 可能机制。方法 将 30 只 6 周龄昆明（KM ）雄性小鼠随机分为三组，以不同剂量硫酸铍生 理盐水溶液腹腔注射染毒，隔日一次，染毒两周。测定其脏器系数；血清中谷丙转氨酶（ALT ）、 谷草转氨酶（AST ）水平；肝脏线粒体肿胀程度；肝脏线粒体中丙二醛（MDA ）含量、谷 胱甘肽过氧化物酶（GSH-Px ）和超氧化物歧化酶（SOD）活性；观察肝脏病理组织学变化。 结果 与对照组比较，硫酸铍染毒组小鼠脏器系数、血清中 AST 和 ALT 水平随剂量增加而 增高，差异有显著性（P0.05 ）；染毒组小鼠肝脏线粒体肿胀明显、肝线粒体 MDA 含量显 著增高（P0.05 ），GSH-Px 活性、SOD 活性随染毒剂量的增加而降低（P0.05 ）；病理组织 学检查可见肝细胞广泛变性、坏死。结论 硫酸铍致小鼠肝毒性作用明显，线粒体脂质过氧 化损伤可能是硫酸铍致肝毒性机制之一。 【关键词】硫酸铍；肝毒性；线粒体；脂质过氧化 Study of Beryllium Sulfate on Mice Hepatotoxicity LUO Jin-jun,LIAO Yong-hong,ZHANG Zhao-hui Wu Duan-sheng. (Institute of Environmental Medicine and Radiation Hygienics ，School of Public Health, University of south China, Hengyang 421001, China) 【Abstract 】Objectiv To study hepatotoxicity and possible mechanism of beryllium sulfate （BeSO4 ）on mice Methods Thirty KM mice were randomly divided into three groups, administered with saline solution of the beryllium sulfate by intraperitoneally injection with every other day for two weeks ．The level of relative organ weight、the activity of serum ALT and AST、 the swelling of mitochondrial 、the content of malondialdehyde （MDA ）、the activity of superoxide dismutase （SOD）and glutathione peroxidase （GSH-Px ）were detected ； Pathological change were examine under optical microscope. Results Compared with the control group, the relative organ weight 、 the activity of serum ALT and AST 、the content of MDA are all increase ，the activity of GSH-Px and SOD are decreased, differences are significant. ；Pathological examination indicated that liver cells were obviously swollen and necrosis widely. Conclusion Beryllium sulf