龙眼多糖超声波_酶解辅助提取工艺优化.pdfVIP

2 0 1 0 5 41 5 DOI: 10. 3969/ .j issn. 1000-1298. 2010. 05. 027 * ) 1 2 2 2 2 2 易 阳 张名位 廖森泰 唐小俊 张瑞芬 魏振承 ( 1. , 430070; 2., 510610) = Box-Benhnken-, pH , :( 8147% ),( 200 /mg) 2 000 /g 250W 55e pH 51 0 60m in, 381 71%, 9185%6141%4135% 31 99%, (P 01 05) : : TS20111 : A : 1000-1298( 2010) 05-0131-06 Optim ization ofU ltrasonic-enzyme-assisted Extraction Technology of Polysaccharides from Longan Pulp 1 2 2 2 2 2 Y iYang Zhang M ingwei Liao Sentai TangX iaojun Zhang Ruifen W eiZhencheng ( 1. College of Food Science and Technology, Huazhong Ag ricultural University, Wuhan 430070, China 2. B io Tech R esearch Institute, GuangdongA cademy of Agricultural Sciences, Guangzhou 510610, China) A stract The effects of the amount of enzym e added, ultrasonic power, enzymolysis temperature, pH value and tmi e on the extraction yield of polysaccharides from longan pulp were optmi ized by response surface methodology. The optmi al technological conditions of extraction by ultrasonic and cellulase were determ ined, then regression mathematicalmodelwas established. The optmi al technological param eters are as follows: longan pulp w ith water ratio is 8147% , cellulase additive ratios is 2000 /g, ultrasonic power is 250W, enzymolysis temperature is 55 e , enzymolysis pH value is 510, enzymolysis tmi e is 60m in, under such conditions, the polysaccharides extraction r