1α%2c25-(OH)2D3通过成骨细胞M-CSF途径及影响破骨细胞增殖分化.pdfVIP

中国畜牧兽医学会家畜内科学分会 中国·南昌 第七届代表大会暨学术研讨会论文集 与分化 顾建红，王世涛，刘学忠，卞建春，袁燕，熊桂林，刘宗平奎 (扬州大学兽医学院，扬州225009) mRNA表达量。M．CSF与骨髓单核细胞共培养9d，抗 la，25-(OH)，D，与OB共培养24、48、72h，采用FQ-PCR法测定M．CSF 酒石酸酸性磷酸酶(TRAP)染色观察并计数OC量，扫描电镜观察象牙片吸收陷窝。【结果】与对照组比较，不同浓度 的表达，问接促进OC的生成和活化，增强骨更新。 关键词l lct,25-(OH)，D3；成骨细胞；破骨细胞：巨噬细胞集落刺激因子 Effectsof on and la，25-(OH)2D3 differentiationM·-CSF ofosteoblast throughpathway Gu Shi—tao，Liu Jian-chun,YuanGui—lin，Liu Jian—hong，WangXue-zhong，Bian Yan，Xiong Zong—ping· 225009) (CollegeofVeterinaryMedicine,YangzhouUniversity,Yangzhou To the ofM-CSF obtainedfrom ratwe糟 investigateexpression mRNA,osteobalstsSpragueDawley Abstract：[ObjectiveI lrealedwith concentrationsof theeffectsofM-CSFOB different Iu,25-(OH)2D3(o1旷10{，107moVL)．Meanwhile，to investigate OrwitbomM-CSE and marrowmononuclear culturedwith osteoelasts’proliferationd／fferentiation,bone ceils(SMM)wcre 【MethodI The ofM-CSFmRNAWgledetermined TheformationofOCwercdetected FQ-PCIL by expression through for lacunar via eleemm histochemieallattrale-resistantacid thedel硎伽of staining phosphatase(TRY)．and resorptionscanning atthe9nI withthecontrol conentrat