旋覆代赭汤对反流性食管炎家兔食管下括约肌L型钙通道的作用研究.pdfVIP

浙江中医药大学学报2014年8月第38卷第8期 旋覆代赭汤对反流性食管炎家兔食管下括约肌 L型钙通道的作用研究 张俊杰 吴茂 申 浙江中医药大学 杭州 310053 摘要：【目的】研究旋覆代赭汤对酸和胆汁混合致反流性食管炎家兔食管下括约肌 Es)L型钙通道的调控机制。[方法】制备家兔反流性食管炎模型，用 硝苯地平(nifedipine)~／．断L型钙通道，比较各组LES环行肌 L型钙通道阻断前后张力幅度的差异。 果]L型钙通道阻断前后各组LES环行肌张力 比较：正常组、全方组、甘升组、去苦降组、去升降相因组LES环行肌钙释放相、内流相张力均高于模型组(尸0，01)，而苦降组、升降相因组、去甘升组 LES环行肌钙释放相与模型组相比无显著性差异(尸0．05)，正常组与全方组LES环行肌钙释放相、内流相张力相比无显著性差异(P0．o5)。[结论】酸 和胆汁混合致反流性食管炎模型家兔LES环行肌张力下降，与L型钙通道功能障碍有关；旋覆代赭汤可以通过调控L型钙通道提高LES环行肌张 力，各拆方组中以甘升组作用显著。 关键词：反流性食管炎；下食管括约肌；平滑肌张力；钙通道；旋覆代赭汤；拆方研究 中图分类号：R331 文献标识码：A 文章编号：1005-5509(2014)08-1007-05 RegulationofL-type Calcium ChannelsbyXuanfu DaizheSoup in theLowerEsophagealSphincterSmooth MuscleofRabbitReflux EsophagitisModelZhangJunjie，WuMaoshenResearchDepartment,ZhejiangChineseMedicalUniversity,Hangzhou(310053) Abstract：o【bjective]ToresearchtheregulationofL—typecalciumchannelsbyXuanfuD~zhesoupinhteloweresophagealsphincter(LES)smoohtmuscle ofrabbitrefluxesophagitismodelinducedbymixedperfusionofhydrochloricacidandbile．[Methods]Establishedhterabbitmodelofmixedreflux esophagitis．difedipinewasusedtoblockL—typecalcium channe1．Themuscletension ofL—typecalcium channelwerecomparedamonggroupsinthe experiments．R[esults]Thecalcium releasingandflowingphaseofLESinhtemodelrgoupWashigherthanthatinhtenormalrgoup，htewholerecipe rgoup，sweet—scendingrgoup，htegettingridofbitter—reducingrgoupandhtegettingridofhftinganddechningrgoup(JPO．01)．Therewasnosignificant differencebetweenthemodelgroupandbitter—reducingrgoup，htehftinganddechningrgoupandthegettingridofsweet—ascendinggroup 0．05)． Therewasnosignificantdifferencebewteenthenormalrgoupandthewholerecipergoupincalcium releasingandflowingphaseofLESfPO．05)． C【onclusion1Thedecreasingofrefluxesopha~tismodelLEStensionwasrelevant、vi出hteL—typecalcium channeldysfunction；XuanfuD~zhesoupcould improvetheLEStensionbyregulatingL—typecalcium channels．Thesweet—ascendinggrouphadaremarkableeffectamongthosedismandepresc邱tion