赤芍中芍药苷提取溶剂的选择.pdfVIP

2006 24 6 Vol24, No6, 2006 Journal of Sichuan of Traditional Chinese Medicine 35 * 赤芍中芍药苷提取溶剂的选择 董明纲 郭春燕 张 力 杨秋红 ( 075000) : 目的: 探讨赤芍中芍药苷提取的最佳溶剂方法: 选用甲醇乙醇和水三种不同溶剂提取, 用YWG-C18 (250 46mm) 色谱柱, 甲醇-005mol/ L 磷酸二氢钾溶液 (3268) 为流动相测定芍药苷的含量, 检测波长为230nm 结果: 在所试浓度 (0010~ 0200g/l) 范围内线性关系良好 r = 09998, n = 5; 甲醇75% 的乙醇重蒸馏水作 溶剂赤芍中芍药苷的含量分别为301% 309% 307% 结论: 结果表明, 甲醇75% 乙醇重蒸馏水作溶剂赤 芍中芍药苷的含量无显著性差异 ( P 005) ; 使用重蒸馏水作溶剂可 避免甲醇较强的毒性且廉价; 我们认为使用重 蒸馏水做溶剂优于乙醇甲醇 : 赤芍 芍药苷 溶剂 HPLC : R2842 : A : 1000-3649 (2006) 06-0035-03 Slection of solvents for the Extraction of Paeoniflorin from Radix Paeoniae Rubra/ Dong Mingg ang Guo Chuny an Zhang L i Yang Qiuhong H ebei N orth University ( Zhangj iakou Hebei 075000) Abstract Objective: To select the optimal solvent for the extraction of paeoniflorin from Radix Paeoniae RubraMethods: Paeoniflorin was extracted from Radix Paeoniae Rubra with methanol, 75% ethanol and redistilled water respectivelyPaeoniflorin was separated on a YWG-C ( 250 46mm) using methanol: 005mol/ L KH PO ( 3268) as a mobile phase and determined at 18 2 4 230nmResults: The linear curve was ideal within the tested range of 0010~ 0200g/ l ( r = 09998, n = 5) ; the average content of paeoniflorin which extracted respectively with methanol, 75% ethanol and redistilled water was 301% , 309% , 307% Conclusions: There was no significant difference in extraction of paeoniflorin with these three solvents ( P 005) , whereas methanol is highly toxic, redistilled water is a low-priced and safe solvent, indicating extraction with redistilled water is better Key words: Radix Paeoniae Rubra Paeoniflorin Solvent HPLC , C18 ( 250 46mm, 10m) ; : -