2011安氏隐孢子虫在HCT_8和AGS细胞中增殖效果的研究.pdf

2011, 27 (6) Chinese Journal of Zoonoses 475 : 1002- 2694( 2011) 06- 0475- 04 安氏隐孢子虫在HCT-8 和AGS 细胞中增殖效果的研究* 1 1 1 2 1 1 1 1 2 逯兆喜 , 吴 亮 ,姜旭淦 , 沈玉娟 ,傅行礼 , 涂国华 ,李 礼 , 陈盛霞 , 曹建平 : 比较安氏隐孢子虫(Cr yp tosp or id ium ander soni ) 在人结肠腺癌( human ileocecal adenocarcinoma, HCT-8) 细胞和人胃腺癌(human stomach adenocarcinoma, GS) 细胞中的增殖 取培养至对数生长期的HCT-8 细胞和 GS 5 细胞, 种于6 孔培养板中, 待细胞生长融合至60%~ 70% 时, 加入2.5 10 个安氏隐孢子虫卵囊, 培养至24 h 和48 h, 以 Giemsa 染色法和Rea-l time PCR 技术检测虫体在细胞中的增殖 Giemsa 染色法观察24 h 和48 h HCT-8细胞中虫体 数量均高于 GS 细胞( P 0. 05) Real-time PCR 技术测得24 h 和48 h HCT-8细胞中安氏隐孢子虫卵囊COWP 基因拷贝 数均高于 GS 细胞( P 0.05) 与 GS 细胞相比, 以HCT-8 细胞作为体外感染模型更利于安氏隐孢子虫的增殖 : 安氏隐孢子虫; HCT-8 细胞; GS 细胞;Giemsa 染色; Rea-l time PCR : R382. 3 : A In vitro proliferation of Cryp t osp orid ium and ersoni in HCT-8 and AGS cell lines LU Zhao-xi, WU Liang,JI NG Xu-gan,SHEN Yu-juan, FU Xing-li,TU Guo-hua,LI Li, CH EN Sheng-xia,C O Jian-ping (S chool of Med ical Sc ience and L a borator y M ed ic ine , J iang su Univ er s ity , Zhenj iang 212013, Ch ina) ABSTRACT:In order to study the proliferation of Cry p tosp or id ium ander soni in human ileocecal adenocarcinoma ( HCT-8) cells and human stomach adenocarcinoma ( GS) cells, HCT-8 and GS cells were harvested at mid-exponential phase and 5 planted in 6 well plates. When the monolayers reached 60%- 70% confluency, the excysted oocysts (2.5 10 / well) were in- oculated into cells and parasite growth was terminated at 24 h and 48 h. Then the proliferation of