Adp53逆转乳腺癌细胞株多药耐药性及其对PgpTOPOⅡ和GSTπ表达的影响.pdfVIP

《中国癌症杂志》2007年第17卷第12期 CHINA0NCOLOGY2007V01．17No．12 Ad—p53逆转乳腺癌细胞株多药耐药性及 11和GST一1T表达的影响 其对P—gP、TOPO [摘要] 背景与目的：肿瘤细胞对抗癌药物产生耐受性是化疗失败的主要原因，突变型p53基因与肿瘤多药 7／Adr多药耐药的逆转作用及其对耐药相关蛋白P-gP、TOPOII和GST·叮r表达的影响。方法：以人乳腺癌MCF一7细 胞及其多柔比星耐药株MCF-7／Adr为实验对象，cck-8法观察Ad-p53对多药耐药的逆转作用，Westernblot检测P- MOI gP、TOPOⅡ和GST一叮r蛋白表达的变化。结果：50 Ⅱ未见明显变化。结论：Ad．p53能够逆转MCF-7／Adr多药耐药，下调P．gP和GST-盯表达。 [关键词]乳腺肿瘤；p53基因；多药耐药；耐药逆转 中图分类号：R73—36文献标识码：A 文章编号：1007—3639(2007)12-0939-03 Theeffectsof onthereversalof resistanceinhumanbreastcancercelllineand Ad-pS3 multidrug its onthe of II and GST·订CHANG impactsexpressionsP-印，TOPO Z协一kun，QIXiao—dong,ZHAO Cancer Medical Guang—zhang,YANGZm—bin(BeiiingTongrenHospital Center，CapitalUniversity，Bering i00730。Chinal Correspondenceto：QIXiao．如馏E-mail：qixiaot@yeah．net and themainobstacleof inthetreat— [Abstract】Background resistance(MDR)is chemotherapy purpose：Muhidrug mentof ithasbeen thatthemuted isrelatedtoMDR．Inthis whether cancer，and reposed p53gene explored study，we adenovirusmediated reverseMDRofhumanbreastcancerandits onthe of p53gene(Ad-p53)could impacts expressions GST一竹．Methods：Inthis humanbreastcarcinoma its P-gP，TOPOⅡand study，adriamycin—resistant cells(MCF-7／Adr)and usedtodeterminetheeffectof was toevaluatethe of parentMdells(MCF-7)were Ad-p53．Cck-8assayadopted cytotoxicity blotwere toobservethe of adriamycin．Westernperformed ex