cfos基因表达与应激性胃黏膜损伤的相关性.docVIP

c-fos基因表达与应激性胃黏膜损伤的相关性 　　作者：宋政军　周晓丽　画伟　苌新明　李长顺 作者单位：西安交通大学医学院第一附属医院消化内科，陕西西安 710061;西安医学院护理系，陕西西安 710021 　　【摘要】目的 探讨c-fos基因表达在心理应激性胃黏膜损伤发生机制中的作用。方法 利用光电刺激仪，建立心理应激动物模型。大鼠随机分为3组，对照组(C)、规则光组(R)和不规则光组(I)。计数胃黏膜损伤指数(UI)，观察胃黏膜组织学变化;采用比色法测定胃黏膜和血液中丙二醛(MDA)的水平;免疫组化法观察胃黏膜中cfos基因的表达情况。结果 ①R组和I组的UI值明显高于对照组(P0.05)，且随着应激时间的延长而逐渐升高;②胃黏膜中cfos表达水平随应激时间的延长而升高，R组与I组明显高于对照组;③cfos表达水平与黏膜MDA含量呈正相关(r=0.693，P0.05)。结论 心理应激性胃黏膜损伤程度随应激时间的延长和严重程度的增加而加重;心理应激下胃黏膜cfos表达可能与氧自由基大量生成有关。 　　【关键词】 心理应激;丙二醛;cfos基因 　　Correlation between cfos gene expression and　gastric mucosa lesion induced by psychological stress in rats 　　Song Zhengjun, Zhou Xiaoli, Hua Wei, Chang Xinming, Li Changshun 　　(Department of Gastroenterology, the First Affiliated Hospital, Medical School of　Xian Jiaotong University, Xian 710061; Department of Nursing,Xian Medical Colloge, Xian 710021, China) 　　ABSTRACT: Objective To study relationship between cfos gene expression and oxygen free radical in gastric mucosa lesion induced by psychological stress in rats. Methods The rats were divided into 3 groups randomly: regular flashing light group (group R), irregular flashing light group (group I ) and control group (group C). The contents of malonaldehyde (MDA), and gastric ulcer index (UI) were measured after stress in different groups. The cfos gene expression in gastric mucous was detected by immunochemistry. Results ① UI increased significantly in group I and group R. ② The content of MDA was increased more significantly than that of the controls. ③ The cfos expression in gastric mucosa was significantly correlated with MDA content (r=0.693, P0.05). And the expression level was increased gradually following the stress time. Conclusion ① Under the psychological stress condition, gastric mucosa lesion is aggravated with the prolonged time. ② The cfos expression in gastric mucosa is correlated with oxygen free radicals. And cfos expression may be induced by oxygen free radicals. 　　KEY WORDS: ps