SA14-14-2株乙型脑炎减毒活疫苗细胞免疫持久性的研究.pdfVIP

SA14-14-2 株乙型脑炎减毒活疫苗细胞免疫持久性研究 李茂光 俞永新 刘欣玉 徐宏山 贾丽丽 王志伟 董关木 ( 中国药品生物制品检定所，北京 100050) 摘要：目的 研究 SA14-14-2 株乙脑减毒活疫苗在小鼠体内的细胞免疫持久性。 方法以SA14-14-2 株乙脑减毒活疫苗0.1ml 皮下免疫BALB/c 小鼠，分别于第14 天、1 个月、3 个月、6 个月和一年处死，取脾脏，ELISPOT 法检测脾淋巴细胞 经乙脑活疫苗抗原刺激后分泌 IFN-γ 和 IL-2 的水平，同时分离血清，混合后检 测中和抗体水平。另一组相同免疫的小鼠于免后各时间点以P3 毒株进行腹腔攻 击，观察疫苗保护效果。结果 乙脑减毒活疫苗免后14 天细胞免疫水平达到高峰， 持续1~3 个月后开始略有下降，免后3 个月至1 年间的脾细胞IFN-γ SFC 和IL-2 SFC 数量仍维持在较高水平。小鼠中和抗体水平在3 个月内由1:20 增加到1:80， 一年内仍维持在1:40。1 年内乙脑减毒活疫苗免疫的小鼠均能很好地抵御乙脑强 毒株的腹腔攻击。结论 SA14-14-2 株乙脑减毒活疫苗能同时诱导细胞免疫反应 和体液免疫反应，细胞免疫至少能维持一年，活疫苗产生的保护效果也至少能持 久一年。 关键词：乙型脑炎减毒活疫苗；细胞免疫；保护效果；免疫持久性 Study on Cellular Immune Persistence of Live Attenuated SA14-14-2 Japanese Encephalitis Vaccine in Mice LI Mao-guang, YU Yong-xin, LIU Xin-yu, et al. (National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, China) Abstract: Objective To study the cellular immune persistence of live attenuated SA14-14-2 Japanese encephalitis vaccine in mice. Methods Live attenuated SA14-14-2 Japanese encephalitis vaccine was subcutaneously injected to BALB/c mice one time. Fourteen days, one month, three months, six months and one year after the immunization, mice were sacrificed, and the spleens were removed aseptically for detection of IFN-γ and IL-2 spot forming cell (SFC) by ELISPOT assay. Serum samples were collected to detect the neutralizing antibody. Another group of immunized mice were challenged by intraperitoneal injection of Japanese encephalitis virus P3 strain to evaluate the protective efficacy at the same time. Results The 作者简介：李茂光（1978-），女，山东省日照市人，中国药品生物制品检定所助理研究员，硕士，研究方 向为病原微生物学。 责任作者：俞永新，E-mail ：yuyongxin@nicpbp.org.cn cellular immunity level reached the peak at 14 days after immunization with the live attenuated JE vaccine and then decreased after 1~3 months. The rates of IFN-γ SFC and IL-2 SFC in spleen cells were maintained at a cer