Ki67和CD44v6在肾癌中的表达及其临床意义.docVIP

Ki-67和CD44v6在肾癌中的表达及其临床意义 　　作者：李威武,李培军,张宁妹　　作者单位：1.宁夏医科大学，银川 750004;2.宁夏医科大学附属医院，银川 750004 　　【摘要】 目的 探讨Ki-67和CD44v6蛋白在肾癌中的表达和临床意义。方法 应用免疫组化方法对40例肾癌组织进行Ki-67和CD44v6蛋白检测。结果 在肾癌组织中Ki-67和CD44v6蛋白表达阳性率分别为65%和35%。Ki-67表达和CD44v6表达与肾癌的病理类型无关(P0.05)，与肾细胞癌分级、分期相关(P0.05)，Ki-67表达与CD44v6表达无相关(P0.05)。结论 Ki-67高表达和肾癌的术后转移相关，CD44v6高表达和淋巴结转移相关。两者对预测肾细胞癌术后转移及淋巴结转移有一定的参考价值。 　　【关键词】 肾肿瘤;Ki-67;CD44v6;免疫组织化学 　　The Clinical Significance and The Expression of Ki-67 and　CD44v6 in Patients with Renal Cell Carcinoma 　　The Affiliated Hosp.of Ningxia Med. Univ.， Yinchuan 750004 　　Abstract：Objective To investigate the clinical significance and the expression of Ki-67 and CD44v6 in patients with renal cell carcinoma and the relationship between them. Methods 40 proteins were detected by Immunohistochemical methods and defined the type of renal cell carcinoma according to the WHO method.Results The expression rates of Ki-67 and CD44v6 were 65% and 35% ，its expression were not related to the type of the renal cell carcinoma (P0.05)，but significantly related to the clinical stage or clinical grade(P0.05). The expression of Ki-67 was not correlated with the expression of CD44v6 (Χ2=2.637，P0.05). The high level expression of Ki-67 were significantly correlated with the postoperative metastasis of renal cell carcinoma. The high level expression of CD44v6 were significantlycorrelated with lymph nodes metastases. Conclusion These results suggest that the high level expression of Ki-67 and CD44v6 might be a good indicator in evaluating postoperative metastasis of renal cell carcinoma and in predicting lymph nodes metastases. 　　Key words：renal cell carcinoma; Ki-67;CD44v6; immunohistochemistry 　　肾癌是常见的泌尿系恶性肿瘤，在泌尿外科肿瘤中占第二位。由于肾癌的转移情况复杂，迄今尚缺乏预测，因此有关肾癌的侵袭和转移已成为目前研究的热点。Ki-67蛋白普遍认为是细胞增殖活性的生物学标志物及研究工具，在泌尿系肿瘤预后及治疗方面的研究应用日益受到关注，目前认为Ki-67的高表达是膀胱癌可靠的独立复发指征[1-2]，但在肾癌方面研究的较少。CD44v6是CD44黏附因子的一种拼接变异体， 其表达常与肿瘤的浸润和转移有关[3]。有关肾癌中联合检测Ki-67和CD44v6蛋白表达的报道很少，本实验采用免疫组化方法检测40例肾癌(RCC)组织、20例癌旁组织和5例正常肾组织中K