NPY在实验大鼠垂体腺瘤中的表达及意义111.docVIP

神经肽Y及其受体在实验性大鼠垂体腺瘤中的表达 陈来照 ( 【摘要】【关键词】Expression of neuropeptide Y (NPY) as well as its receptors in estrogen-induced pituitary adenoma in rats CHEN Lai-zhao﹡, MA Jing-jian. ﹡Department of Neurosurgery, First hospital，Shan Xi Medical University, TanYuan 030001, China。 【Abstract】Objective The aim of this study was to explore the expression of neuropeptide Y as well as its receptors in estrogen-induced pituitary tumors of Wistar rats, to investigate their roles in pituitary tumorigenesis. Methods 40 female Wistar rats were divided randomly into experimental and control groups. The rats were induced with estradiol benzoate by peritoneal injection in experimental group. The expression of NPY as well as its receptors in pituitary glands was also detected by immunohistochemical analysis and semi-quantitative reverse transcription polymerase chain reaction (RT-PCR). Some pituitary tissues were also examined using immuno-electron microscopy in order to study the distribution of NPY at the subcellar level. Results (1) In experimental group, significant increases in pituitary weight were noted three months after peritoneal injection of estrogen. The serum prolactin concentration also increased sharply. (2) The expression level of NPY in pituitary of experimental groups were significantly lower compared to that of control group (P 0.001). RT-PCR detection also indicated that the gene expression of Y1 and Y2 recepors were decreased in the pituitary tumors (P 0.001). (3) Immuno-electron microscopy examination demonstrated that positive NPY staining with a high density of gold particles located mainly in the secretory granulas. Conclusion These data suggest that chronic treatment with estrogen can result in pituitary adenoma in female Wistar rats. Eexpression of NPY as well as its receptors (Y1R and Y2R) was decreased by estrogen in the pituitary glands, with possible involvement in estrogen induced pituitary tumorigenesis in rats. 【Key words】Ratituitary adenoma model; Neuropep