TF_1细胞凋亡相关基因的研究_刘红涛.pdfVIP

·38 · 　　Prog.Biochem.Biophys.　　　　　1999;26 (1) Construction, Expression and Purification of 　　　 Calponin T184A:a Mutant of Calponin.CAO 1 　, , (Tang D C, Xiang J Z, Lu W T). Sheng, TANG Da-Chun, LUO Jian-Yun (Key Calponin. (Prog Biochem Biophys), 1996, 23 (4):32 ～329 Laboratory of Pulmonary Disease, Health Ministry 2 　Tang D C, Kang H M, Jin J P, et al.Structure-function relations of China;Department of Pathophysiology , Tongj i of calponin.Biophys J, 199 , 68 (2Pt2):A163 3 　　, , (Cao S, Tang D C, Luo J Y). Medical University , Wuhan 430030, China). . (Acta Abstract　Calponin cDNA was site-mutated (Thr184 Universitatis Medicinal Tongji), 1997, 26 (1):67 ～68 4 　J, E F, T (Sambrook J, Fritsch E ※Ala184 )by overlap extension PCR method to F, Maniatis T )., . (Molecu- reduce the opportunity for calponin to be phosphory- lar cloning:a laboratory manual). (Beijing): (Science Press), 1992.23 ～371 lated by PKC or CaM K Ⅱ.The PCR product was 　Winder S J, Walsh M P.Calponin:thin filament-linked regulation sequenced.The mutated cDNA w as then recombined of smooth muscle contraction.Cell Signal, 1993, 5 (6):677 ～ into an expression vector pAED and transferred into, 4 682 6 　Sobue K, Sellers J R.Caldesmon:a novel regulatory protein in expressed in .coli BD (DE )induced by IPTG. 21 3 smooth muscle and nonmuscle actomyosin systems.J Biol Chem, 1991, 266 (19):1211 ～ 12118 The expression level was up to 1 %of total bacterial 7 　Tang D C, Stull J T,