丙戊酸对肺癌细胞系A549增殖和凋亡影响的研究.pdfVIP

∀ 894 ∀ , A549 实验研究 A549 1, 2 1 1 2 1 1 姜明来 , 张开基 , 李辉 , 李青云 , 邹志, 李万成 1. 泸州医学院第 一附属医院呼吸内科, 四川泸州646000 2. 焦作市第二人民医院肿瘤内科, 河南焦作454150 Depressent effect of VPA on proliferation and apoptosis of human lung cancer cell lineA549 1, 2 1 1 2 1 1 JIA NG Minglai , ZHA NGK aij i , LIH ui , LI Qingyun , ZOUZhi , LI Wancheng 1. Dep art ent of Resp iratory, Af f iliated H osp ital of Luzhou Medical College, Luzhou 646000, P.R. China 2. Dep art ent of Oncology, Second Peop le s H osp ital of Jiaozuo, Jiaozuo 454150, P. R. China ! : 探讨丙戊酸( valproic acid, [ ABSTRACT] OBJECTIVE: o invest igate t he rule of VPA on VPA) 抑制肺癌细胞系 A549 增殖和凋亡 the proliferation and apoptosis of human lung cancer cell 作用: 将 05~ 20 mmol/ L 丙戊酸 lineA549. METHODS: Cultured A549 cells were treated by VPA 钠作用于 A549 细胞48 h, 观察细胞数量和 at the concentrations of 05, 10 and 20 mmol/ L for 48 hours. 形态 变化, 并用 M 法分析细胞生长抑 he morphological and quant changes of A549 cells treated by 制, 流式细胞仪测定细胞周期动力学变化, VPA w ere observed through a microscope and the proliferation w as 细胞免疫组化测定Caspase3: VPA predicted by methyl thiazolyl tetrazolium ( M ) . he expression 干预后细胞数量明显减少, 形态不规则, 细 of Caspase3 w as detected by immunocytochemistry. he change 胞核固缩, 胞质减少; 与对照组比较, VPA of cell cycle was detected by flow cytometry. RESULTS: he popula 可造成 A549 细胞生长抑制, F= 28312, tion of the cells decreased and the shape and size changed after being P 005且随着 VPA 浓度 增加, 抑制 treated w ith VPA. VPA could inhibitate the growth of A549 signifi 率增加; G 期