包埋玻璃化法超低温保存沙生柽柳休眠茎尖的研究.pdfVIP

, Journal ofAnhuiAgr.i Sci. 2010, 38( 11) : 5576- 5577, 5607 刘月娟 况玲玲 刘艳萍, 滕立平, 段黄金, 鲁乃增, 王高辉 (, 843300) [目的]采用包埋玻璃化法, 对沙生柽柳休 茎尖成功地进行冷冻保存[方法]将沙生柽柳休 茎尖包埋在含 3%褐藻酸钙的胶 球中, 研究了预培养中蔗糖浓度培养时间和不同玻璃化保护液处理的时间对沙生柽柳休 茎尖存活率的影响[结果]含沙生柽柳茎 尖的胶球在 0. 8mol/L蔗糖溶液中培养 24 h可获得较高的存活率; 胶球经玻璃化保护液[ 40% 甘油+ 45% ( 0. 4 mol/L)蔗糖+ 10%聚乙 二醇(分子量 4 000) + 5%二甲基亚砜]在 25 处理 20 m in可以明显提高存活率, 在优化条件下存活率可高达 48. 3% [结论] 蔗糖浓 度和预培养时间是决定沙生柽柳茎尖存活率的关键因素 沙生柽柳; 休 茎尖; 超低温保存; 包埋玻璃化法 S793. 5 A 0517- 6611( 2010) 11- 05576- 02 Cryopreservation of Shoottips ofTam ar ix tak lamakanens is Domant BudsbyEncapsulationvitrification LIU Yanping et al ( Coll g of P lant Sci nc , T ar mi U niv rsity, A lar, X injiang 843300) Abstract [ Obj ctiv ] Shoottips ofTam ar ix taklamakanensis D omant buds w r succ ssfully cryopr s rv d by using ncapsulationvitrification. [M thod] Domant shoottipsw r ncapsulat d 3% calcium alg inat b ads. Th main factors influ ncing th v iability such as sucros conc ntra tion, pr cultur tmi , d iff r nt v itrification solution and th tmi b ads in v itr ification solution w r studi d. [ R sult] H igh viability could b ob tain d wh n alginat b ads w r pr cultur d for 24 h in 0. 8 m ol/L sucros. Th b ads w r d hydrat d w ith v itrification solution [ 40% Gly + 45% ( 0. 4 mol/L) Suc + 10% PEG ( 4 000) + 5% DMSO] for 20 m in at 25 . U nd r optmi um conditions, th v iability of domant shoottips could r ach 48. 3% at th most. [ Conclusion] Sucros conc ntration and pr cultur tmi w r mi portant factors influ ncing th v iability ofT ama rix taklam akanensis D omant buds. Key ords Tam ar ix taklamakanensis Domant; Shoottip; Cryopr s rvation; Encapsulationvitrification ( Tamar ix tak lamakanens is) 1. 2 3% , , 10. 1mol/L CaCl 2 , 30m in, 4 mm [ 1- 2] ,