表面微纳米沟槽结构对成纤维细胞黏附和骨架重排的促进作用.pdfVIP

28 6 Vol. 28 No. 6 2009 12 Chinese Journal of Biomedical Engineering December 2009 1 1 1 2* 1* 齐晓谨 孟 洁 孔 桦 吴晓春 许海燕 1( , 100005) 2( , 100080) : , , , , , , , , : ; ; ; Micronano Groove Patterns Inducing Fibroblast Cells Adhesion and Cytoskeleton Rearrangement 1 1 1 2* 1* QI XiaoJin MENG Jie KONG Hua WU XiaoChun XU HaiYan 1 ( Institute of Basic Medical Sciences, ChineseAcademy of Medical Sciences Peking UnionMedical College , Beji ing 100005) 2 ( National Centerf or Nanoscience and echnology , Beij ing 100080) Abstract: Surface topography is one of the potential important factors for biomaterials, which influences cells behaviors such as cell a hesion, proliferation, ifferentiation an even apoptosis. It is clear that cells woul respon to nanometric scale surface features, therefore it is possible to in uce certain responses of cells by fabricating substrates with combine micronano patterns. Herein, transparent polyurethane films with micronano groove patterns were prepare by solventcasting from a template. Mouse fibroblast cells ( 3T3L1) were see e on the patterning films an cultivate for at least one week. Cell morphologies an cytoskeleton protein expression in the fibroblasts were observe with optical microscope an confocal laser scanning microscopy. Cells a hesion an proliferation were examine using MTS assay. Experimental results showe that themicronano groove pattern significantly enhance the a hesion an proliferation of fibroblasts. The mi