阳离子交换树脂酸水洗脱法纯化苦参总碱的工艺优选.pdfVIP

齐鲁药事 ·Qilu Pharmaceutical Af f airs 2008 Vol27 , N o 10 ·62 1 · 阳离子交换树脂酸水洗脱法纯化苦参总碱的工艺优选 代龙 ( 山东中医药大学 ,山东 济南 250355) 摘要 : 目的　优选阳离子交换树脂不同浓度盐酸溶液洗脱纯化苦参总碱的最佳工艺条件 。方法 　以苦参碱 、氧化苦参碱 和总生物碱的洗脱率为考察指标 , 以盐酸溶液作为洗脱剂 ,优选阳离子交换树脂纯化苦参总碱的最佳工艺和参数条件 。结果 　苦参总碱阳离子交换树脂纯化洗脱条件为 : 以5 %盐酸溶液作为洗脱剂 , 以 6 BV ·h - 1 的流速洗脱 ,苦参碱和氧化苦参碱 、 苦参总碱洗脱率均可达 85 % 。结论 　采用阳离子交换树脂酸水洗脱法纯化苦参总碱效率高 ,工艺简便 ,可用于苦参总碱的提 取纯化 。 关键词 :阳离子交换树脂 　苦参总碱 　纯化工艺 ( ) 中图分类号 :R2842 　文献标识码 :A 　文章编号 :1672 - 7738 2008 10 - 062 1 - 03 Optimization of purifying process of total al kaloids from sophora flavescens by cation exchange ion resin with hydrochloric acid DA I Lo ng ( Shandong U niver sit y of Traditional Chinese Medicine ,J i′nan 250355) ABSTRACT :OBJECTIVE 　To select t he op timum p urificating p rocess of tot al alkaloids in sop hora flavescen s by mean s of cation exchange ion resinMETHODS 　The p urificating p rocess of tot al alkaloids in sop hora flavescen s was p erformed by cation exchange ion resin wit h t he solvent of hydrochloric acid ,and t he cont ent of mat rine ,oxymat rine and tot al alkaloids in sop hora flavescen s were det ermined to choo se t he op timal t echnological p aramet er s RESUL TS 　The ideal solvent i s 5 % hydrochloric acid ,and t he op tima flow rat e i s 6BV ·h - 1 The cont ent of mat rine ,o xymat rine and tot al alkaloids washed off f rom cation ex change ion resin are all above 85 % CONCL USION 　The p urification p rocess wit h 5 % hydrochloric acid as rep la