牙根发育不全疾病致病差异表达基因文库的建立.pdfVIP

2003 10 19 10 J Clin Stomatol, Oct, 2003, Vol. 19, No. 10 5 7 * 1 2 1 2 1 2 3 轩 昆, 金 岩 , 文玲英 , 李 鑫 , 杨富生 , 郑 梁 , 金 明 ( 1. 第四军医大学口腔医学院儿童口腔科 陕西 西安 710032; 2. 组 病理学教研室; 3. 生物化学与分子生物学教研室) [ ] : cDNA : , mRNA , cDNA, pGEM- T T/ A , , 100 : cDNA 400 , 100 90% : cDNA , [ ] ; ; [ ] Q7 6 [ ] A [ ] 1003- 1634( 2003) 10- 05 7- 04 Construction of cDNA library subtracted f rom congenital hypoplasia of teeth roots XUAN Kun , JIN Yan, WEN Ling- ying, LI Xin, YANG Fu- sheng , ZHENG Liang, JIN Ming. department of pediatric dentistry , college of stamotol ogy, fourth military medical university, Xi an 710032, China [ Abstract] Objective:To construct the differentially expressed cDNA library subtracted from the pat ient of congenital hypoplasia of teeth roots and normal brother. Method: By modified subtractive hybridization technique, total RNA sample of Peripheral venous blood extracted from the patient of congenital hypoplasia of teeth roots and normal brot her were sub tracted to screen the differentially expressed cDNA of candidate virulence gene related w ith t his disease. Then the cDNA were ligated with pGEM- T vector, and the inserted vector were transformed to competence Escherichia coli for library amplification. 100 w hite clone w ere selected randomly and evaluate by enzyme digestion. Result: 400 white cloning w ere obtained in the cDNA library, and after enzyme digestion there were inserted fragmentsin 90% of 100 cloning selected randomly. ConclusionThe differentially expressed cDNA library was construc