黄芪中黄芪甲苷的提取工艺.pdfVIP

黄芪中黄芪甲苷的提取工艺 一研论～ ～究文一 杨克迪，李 宏，王丽君 (广西大学化学化工学院，广西南宁530004) 摘要：采取乙醇回流法，研究了不同浓度乙醇、溶媒量、提取时间和提取温度对黄芪中黄芪甲苷提取的影 响，通过正交法和单因素法优化了黄芪中黄芪甲苷的最佳提取工艺；应用固相萃取与高效液相联用技术测 定黄芪甲苷的含量，方法简便准确。结果表明，黄芪中黄芪甲苷的最优提取方法为取15倍55％的乙醇在 80℃下回流提取4h。本实验优选出的工艺科学、合理，为黄芪甲苷的提取条件提供了参考依据。 关键词：黄芪；黄芪甲苷；固相萃取I高效液相色谱 中图分类号：R284．2 文献标识码：A oftheextractionof N Study Astragaloside in membranaceus Astragalus YANGKedi，LI Lijun Hong，WANG andChemical GuangxiUniversity·harming (CollegeofChemistry Engineering tOthe and test，Fourfactorsinextractionof Abstract：Accordingorthogonaldesignsingle-factor Astragalo— methodofethanol concentration membranaceuswere withthe reflux，i．e．the sideⅣinAstragalus optimized of solvent durationand contentof ethanol，theamountof added，extractingtemperature．TheAstragaloside IVwasdeterminedthecombination ofsolid extractionand chro— by technologyphase highperformanceliquid was andaccurate．Theresultshowedthatthebestextractionof matography，whichsimple processAstragalo。 80Cin15timesamountof for4 membranaceuswasextractedrefluxof 55％ethanol sideⅣinAstragalus conditionof IVin membranaceuswas withhe hours．The optimizedorthogonal extracting AstragalosideAstragalus and test，itisreasonableandscientific． designsingle-factor