黄芩甙对脑细胞老化作用的实验的研究.pdfVIP

·临床与实验研究· 黄芩甙对脑细胞老化作用的实验研究 1 2 路雪雅 陈惟昌 【摘要】目的 本文以抗坏血酸和硫酸亚铁为诱导脑细胞老化的实验模型。研究黄芩甙对其脑细 胞损伤的作用。方法 采用分光光度法检测脑细胞脂质过氧化物和线粒体膨胀，氧电极法测定脑细胞 耗氧量。结果 实验结果显示：脑细胞和线粒体分别在抗坏血酸和硫酸亚铁的作用下，脂质过氧化物升 高，线粒体膨胀。黄芩甙具有明显的降低其脑细胞脂质过氧化的生成和线粒体膨胀。不同浓度的黄芩 甙与脂质过氧化物生成量具有较高的相关性(r=0.78)，直线回归方程 y=4.885〒2.951 X，X=1.089 〒0.301 Y.黄芩甙降低线粒体膨胀以反应60min 为例，由诱导组的光密度(0.214〒0.023)分别增加 到(0.244〒0.041)，(0.281〒0.019)和(0.371〒0.019)。黄芩甙降低脑细胞耗氧量也具有显著的 统计学差异。结论 黄芩甙具有防止抗坏血酸和硫酸亚铁诱导脑细胞结构和功能的老化作用。 【关键词】黄芩甙 脂质过氧化物 线粒体膨胀 脑细胞耗氧 Experiment Study of Baicalin on the Injury of Brain Cell Effect. 1 2 Lu Xueya ,Chen Weichang .1.Department of Biochemistry, Beijing University of Traditional Chinese Medicine, Beijing 100029 China; 2.Institute of clinical Medicine of China-Japan Friendship Hospital 【Abstract 】Objective AIM The experiment model was used of the injury of the rat brain cell induced by ascorbate and FeSO4. It has been studied that effect of baicalin on this injury of the rat brain cell. Methods The brain mitochondria swelling and the lipid peroxidation were detected by the method of spectrophotometer. Oxygen consumption was measured by oxygen electric.Results The mitochondria swelling and increased of lipid peroxides were shown in rat brain mitochondria and cell induced by ascorbate-FeSO4. The baicalin decreased significantly of the mitochondria swelling and the production of lipid peroxidation in brain cells induced by ascorbate-FeSO . The baicalin decreased the mitochondria swelling, as exsample of the reaction 4 60min, absorbance (520nm) of (0.214 〒0.023)in induced injury group increased to (0.244 〒0.041) (P ＜ 0.05),(0.281 〒0.019)(P ＜0.05)and (0.371 〒0.019) (P ＜0.01). The baicalin with lipid peroxid