48个油橄榄品种的遗传多样性及聚类分析（英文版）.pdfVIP

Agricultural Science Technology, 2013, 14(9): 1215-1219 Copyright 訫 2013, Information Institute of HAAS. All rights reserved Molecular Biology and Tissue Culture Genetic Diversity and Clustering Analysis of 48 Cultivars of Olea euyopaea L. 1 2 鄢 1 鄢 2 1 2 2 Delu NING , Shaoyu CHEN , Haiyun CHEN , Rui LI , Yongjie LI , Yunling MAO , Tao WU 1. Institute of Economic Forest Trees, Yunnan Academy of Forestry ，Kunming 650201, China; 2. Key Laboratory of Forest Plant Cultivation and Utilization of Yunnan Province, Yunnan Academy of Forestry / Yunnan Labora鄄 tory for Conservation of the Rare, Endangered and Endemic Forest Plants, State Forestry Administration, Kunming 650201, China Abstract Inter鄄simple sequence repeat (ISSR) molecular markers were applied to an鄄 48 个油 橄 榄 品 种 的 遗 传 多 样 alyze the genetic diversity and clustering of 48 introduced and bred cultivars of Olea euyopaea L. Totally 106 DNA bands were amplified by 11 screened primers, includ鄄 性及聚类分析 ing 99 polymorphic bands; the percentage of polymorphic loci was 93.40%, indicat鄄 宁德鲁 1 ，陈少瑜 2* ，陈海云 1* ，李瑞 2 ，李勇杰 1 ， ing a rich genetic diversity in Olea euyopaea L. germplasm resources. Based on ’ 毛云玲 2 ，吴 涛 2 （1. 云南省林业科学院 ，云 Nei s genetic distances between various cultivars, a dendrogram of 48 cultivars of （ ） Olea euyopaea L. was constructed using unweighted pair鄄group UPMGA method, 650201 2. / 南昆明 ； 云南省林业科学院 云南省 which showed that 48 cultivars wer