《A Fragment of CadherinLike Protein Enhances Bacillus thuringiensis Cry1B and Cry1C Toxicity to Spodoptera exigua Lepidoptera Noctuidae》.pdfVIP
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. ’5“≯4掣 2012
1 1 628
¨署10裟把铲篓iveA掣沁ulm代. f41: 638 April
飘飘丽■霹霪黍黧厂————一 一
ProteinEnhancescillus B
A ofCadherin—Like thuringiensisCryl
Fragment
and C to
CrylToxicitySpodoptera
Xi—wu‘andGUO
Kong—min92.GAO Yu—yuan2
and 100193,P.R.China
University,Beijing
,CollegeofAgricultureBiotechnology,ChinaAgricultural
2 PlantDiseasesandInsectPests/InstituteProtection,Chinese
StateKey o|Plant AcademyofAgricultural
LaboratoryforBiologyof
lOOl93,P,RChina
Sciences,Beijing
Abstract
effective insecticidescertaininsect
toxins Bacillus species.In
Cry by thuringiensis(Bt)arebiological against
produced
this resultsindicatedthat lBand 1 Cweretoxicto alsoidentifieda
study.bioassay Cry Cry Spodopteraexigua.We
the of and cadhefin—likeidentifiedfrom
cadherin.1ikeinS thatcouldenhance
gone exigua toxicityCrylBCrylC.The gone
thelarvae tissuewasclonedreverse chain
polymerasereaction(RT-PCR)andrapidamplification
midgut by transcription
ofeDNA eDNAofthe consistedof5220 1 740aminoacidwitha
ends(RACE).Thefull.1ength gene bpencoding predicted
acid hada
massof196kD,BLASTsearch showedthatthe
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