提高不对称还原合成_R_扁桃酸的效率.pdf

化 工 进 展 ·1792 · CHEMICAL INDUSTRY AND ENGINEERING PROGRESS 2009 年第 28 卷第 10 期 研究开发 提高不对称还原合成（R ）-扁桃酸的效率 韩 莹，穆晓清，徐 岩 （江南大学生物工程学院教育部工业微生物重点实验室，江苏无锡 214122 ） 摘 要：以葡萄酒酵母Saccharomyces ellipsoideus 为催化剂，对提高不对称还原苯乙酮酸生产(R)-扁桃酸效率进行 了研究，分别利用了分批补料、添加吸附树脂的方法降低底物对反应过程的抑制作用，提高反应的底物浓度。并 且通过对吸附树脂吸附性能的考察，筛选出了一种对底物有较快吸附速率和较大吸附量的树脂NKA Ⅱ，可以显著 降低底物对反应过程的抑制，提高反应的底物浓度。最终发现将两种方法结合，在添加吸附树脂的体系中进行分 批补料是最高效的方法，将底物浓度从33.31 mmol/L 提高到166.53 mmol/L，并且产率达到了91.6% ，e.e.值为100%， 而且菌体重复使用性能很好。 关键词：葡萄酒酵母；不对称还原；底物抑制；吸附树脂；(R)-扁桃酸 中图分类号：TQ 921 文献标识码：A 文章编号：1000 –6613 （2009 ）10 –1792 –06 Enhancement of asymmetric reduction efficiency for (R)- mandelic acid synthesis HAN Ying ，MU Xiaoqing ，XU Yan （Key Laboratory of Industrial Biotechnology of Ministry of Education ，Jiangnan University ，Wuxi 214122，Jiangsu，China ） Abstract ：Enhancement of asymmetric reduction efficiency for (R)-mandelic acid (MA) from phenylglyoxylic acid (PGA) was investigated with whole cells of Saccharomyces ellipsoideus as catalyst. Utilization of fed-batch and addition of adsorbent resin could be effective in increasing substrate concentration and mitigating substrate inhibition. Absorbent resin NKA Ⅱ was selected ，which had a large adsorption amount and a high adsorption rate of the substrate. By adding NKA Ⅱ adsorption resin in the reaction system，substrate inhibition was mitigated，and the initial substrate concentration was thus increased. Finally it was found that combining these two methods was the most effective way. Substrate concentration was increased from 33.31 mmol/L to 166.53 mmol/L, and a 95.6% yield of (R)-mandelic was obtained with fed-batch in a resin-added sy