响应面法优化丹参叶总酚酸超声波辅助提取工艺.pdfVIP

34 2011, Vol. 32, No. 12 食品科学 ※工艺技术 响应面法优化丹参叶总酚酸超声波辅助 提取工艺 薛治浦，李 欣，朱文学 (河南科技大学食品与生物工程学院，河南洛阳 47 1003) 摘 要：对丹参叶总酚酸的超声提取工艺优化进行研究。在单因素试验基础上，选择提取溶剂乙醇体积分数、提 取时间、温度和液料比为自变量，以丹参叶总酚酸得率为响应值，采用Box -Behnk en 试验设计方法，研究各自变 量及其交互作用对丹参叶总酚酸提取率的影响。利用Design Expert 软件得到回归方程的预测模型并进行响应面分析， 确定超声波辅助提取丹参叶总酚酸的最佳条件为乙醇体积分数63 .00% 、浸提时间4 3 .00min 、温度50 .00 ℃、液料 比3 3 : 1(m L/ g ) ，在此条件下，总酚酸提取率达到7 .7 8% 。验证实验表明，所得模型方程能较好地预测实验结果。 关键词：丹参叶；总酚酸；超声波；响应面 Optimization of Ultrasound-assisted Extraction of Total Phenolic Acids from Salvia miltiorrhiza Bunge. Leaves Using Response Surface Analysis XUE Zh i-pu ，LI Xin ，ZHU Wen-xue (College of Food and Bioengineering, Henan University of Science and Technology, Luoyang 47 1003, China) Abstract ：Response surface methodology was used to optimize the ultrasound-assisted extraction of total phenolic acids from Salvia miltiorrhiza Bunge. Leaves. The extraction efficiency of total phenolic acids was investigated with respect to four variables including ethanol concentration, time, temperature and liquid-to-solid ratio. On the basis of a series of one-factor-at-a-time experiments, a polynomial regression model equation was fitted by the combined use of Box-Behnken experimental design and regression analysis. By analyzing the regression model using response surface analysis, the optimum extraction conditions of total phenolic acids from Salvia miltiorrhiza bunge. Leaves.were identified as follows: 63% ethanol concentration, 43 min extraction time, 50.00 ℃extraction temperature and 33:1 (mL/g) liquid-to-solid ratio, and the extration efficiency of total phenolic acids was up to 7.78% under the optimized conditions. Confirmatory exp