发光二极管红光对实验性高脂血症大鼠血脂的调节作用.pdfVIP

中国康复理论与实践2013 年9 月第19 卷第9 期 Chin J Rehabil Theory Pract, Sep. 2013, Vol. 19, No.9 - 824 - DOI : 10.3969/j.issn. 1006-9771.2013.09.007 ·基础研究· 发光二极管红光对实验性高脂血症大鼠血脂的调节作用① 卢建丽，刘翠霞，尹昱，安经克，贾子善 [摘要] 目的 探讨发光二极管(LED)红光照射对高脂血症大鼠血脂的调节作用。方法 健康雄性Sprague-Dawley 大鼠36 只随 机分为正常对照组(n= 12)和高脂模型组(n=24) 。正常对照组饲喂普通饲料，高脂模型组采用高脂饲料喂养6 周。造模成功后，再将 高脂模型组随机分为高脂对照组(n= 12)和LED 治疗组(n= 12) 。高脂对照组不给予照射，LED 治疗组给予LED 红光照射，波长(630± 15) nm ，每天30 min ，连续照射28 d 。生化法检测大鼠血清总胆固醇(TC)、甘油三酯(TG)、低密度脂蛋白(LDL-C)、高密度脂蛋白 (HDL-C)水平及脂蛋白酯酶(LPL)和肝脂酶(HL)活性。肝脏组织免疫组化染色观察羟甲基戊二酰辅酶A 还原酶(HMG-CR) 的表达。 结果 LED 治疗组大鼠血清TC 、TG 和LDL-C 水平较高脂对照组明显减低(P0.01) ，而HDL-C 水平明显升高(P0.01) ；LED 治疗组 血清LPL 和HL 的活性明显高于高脂对照组(P0.01) ，LED 治疗组肝脏组织中HMG-CR 的表达低于高脂对照组(P0.05) 。结论 LED 红光照射通过增强LPL 、HL 的活性及降低HMG-CR 的表达，影响血清脂质的代谢，从而达到调节血脂的作用。 [关键词] 红光照射；高脂血症；脂蛋白酯酶；肝脂酶；羟甲基戊二酰辅酶A 还原酶；大鼠 Effect of Light Emitting Diode Red Light Irradiation on Hyperlipidemia Rats LU Jian-li, LIU Cui-xia, YIN Yu, et al. Department of Rehabilitation, Hebei General Hospital, Shijiazhuang 050051, Hebei, China Abstract: Objective To observe the effect of light emitting diode (LED) red light irradiation on serum lipid in experimental hyperlipid- emia rats. Methods 36 healthy male Sprague-Dawley rats were randomly divided into normal control group (n= 12) and hyperlipidemic mod- el group (n=24). The normal group was fed with normal diet while the hyperlipidemic model group with fat-rich forage for 6 weeks. The hy- perlipidemic model group rats were randomly divided into the hyperlipidemic control group (n= 12) and LED treatment group (n= 12), and the latter accepted LED red light irradiation for 28 d. The levels of serum lipid including total cholesterol (TC), triglyceride (TG), low-densi- ty lipoprotein (LDL-C), high-density lipoprotein (HDL-C), and the activities of lipoproteinesterase (LPL