枯草芽孢杆菌BAB_1产脂肽类及挥发性物质的分离与鉴定.pdfVIP

中国农业科学 2010,43(17):3547-3554 Scientia Agricultura Sinica doi: 10.3864/j.issn.0578-1752.2010.17.008 枯草芽孢杆菌BAB-1产脂肽类及挥发性物质的分离和鉴定 李宝庆，鹿秀云，郭庆港，钱常娣，李社增，马 平 （河北省农林科学院植物保护研究所/河北省农业有害生物综合防治工程技术研究中心，河北保定 071000 ） 摘要：【目的】对枯草芽孢杆菌Bacillus subtilis 菌株 BAB-1 产生的脂肽类物质和挥发性物质进行分离鉴 定和抑菌活性研究。【方法】采用HPLC和MALDI-TOF-MS对脂肽类物质进行分离纯化和鉴定；采用GC-MS对挥发性 物质进行鉴定。【结果】HPLC 分析表明，菌株BAB-1产生的脂肽类物质由LP-1、LP-2、LP-3三类组分组成，其中 LP-2 起主要抑菌作用。MALDI-TOF-MS 质谱分析表明，LP-1 为 surfactin（C13—C15），LP-2 为 fengycin A（C14 —C15）和fengycin B（C14—C18），LP-3成分未能确定。GC-MS结果表明，菌株BAB-1 产生的挥发性物质由多种 成分组成，主要为醇类、酮类、酸类、胺类和烷烃类等。【结论】枯草芽孢杆菌菌株BAB-1能够产生脂肽类物质和 挥发性物质，并且这两类物质都具有较强的抑菌活性。 关键词：枯草芽孢杆菌；脂肽；挥发性物质；抑菌活性 Isolation and Identification of Lipopeptides and Volatile Compounds Produced by Bacillus subtilis Strain BAB-1 LI Bao-qing, LU Xiu-yun, GUO Qing-gang, QIAN Chang-di, LI She-zeng, MA Ping (Institute of Plant Protection, Hebei Academy of Agricultural and Forestry Sciences/IPM Centre of Hebei Province, Baoding 071000, Hebei) Abstract: 【Objective 】 The objective of this study was to isolate and identify the lipopeptides and volatile compounds produced by Bacillus subtilis strain BAB-1, and to analyze their antifungal activities. 【Method 】 Lipopepetide compounds were isolated, purified and identified by using HPLC and MALDI-TOF-MS techniques, and the volatile compounds (VCs) were identified by using GC-MS method. 【Result 】 Three fractions of the lipopeptides, LP-1, LP-2 and LP-3, were collected, among which LP-2 showed the major antifungal activity. According to the mass spectra analysis, LP-1 was determined to be identical to surfactin, varying in the length of the fatty acid chain moiety from C13 to C15.