TLC测定冬凌草叶中冬凌草甲_乙素的含量.pdf

TLC 测定冬凌草叶中冬凌草甲、乙素的含量 袁珂 　胡润淮 　杨怡 　孙伟 　张晓明 (郑州 450003 河南中医学院中药系) 摘要 　目的:研究冬凌草叶中冬凌草甲、乙素的含量测定 。方法 :采用薄层扫描法进行测定 。结果 :冬凌草甲素浓 μ μ 度在 9 . 50 g～47 . 50 g 之间线性关系良好 ,相关系数 r = 0. 999 , 回收率为 98. 54 % ,测定的 R SD = 1. 4 1 % 。冬凌 μ μ 草乙素浓度在 9 . 98 g～49 . 90 g 之间线性关系 良好 ,相关系数 r = 0. 999 , 回收率为 98. 07 % ,测定的 R SD = 1. 35 % 。结论 :本法简便 、准确 、灵敏 ,重现性好 ,可用于冬凌草的质量控制 。由本法测定结果表明 ,生长在济源太 行山区 8 月份的冬凌草叶中冬凌草甲、乙素的含量最高 。 关键词 　冬凌草 ;冬凌草甲素 ;冬凌草乙素 ;薄层扫描法 TL C in quantitative determination of oridonin and ponicidin in the leaves of R abdosi a r ubescens Yuan Ke ( Yuan K) , Hu Runhuai ( Hu RH) , Yang Yi ( Yang Y) , et al ( He ’nan College of Traditional ) Chinese M edicine , Zhengz hou 450003 ABSTRACT 　OBJECTIVE : To study t he met hod for quantitative determination of oridonin and ponicidin in t he leaves of R abdosia rubescens . METHODS : TL CS met hod was selected to determine t he content . RESUL TS : For oridonin t he μ μ linear range was 9 . 50 g ～47 . 50 g wit h r = 0 . 999 , t he average recovery was 98. 54 % wit h R SD 1. 4 1 %. For μ μ ponicidin t he linear range was 9 . 98 g ～49 . 90 g wit h r = 0 . 999 , t he average recovery was 98. 07 % wit h R SD 1. 35 %. CONCL USION : The met hod was proved to be simple , precise sensitive and reproduciable . It can be used for t he quality control of R abdosia rubescens . The result s showed t hat t he content of oridonin and ponicidin in t he leaves of R abdosia rubescens , which grows in August in J i Yuan county area , is relatively t he highest . KEY WORDS 　R abdosia rubescens , oridonin , ponicidin , TL Cscanning ( ) ( 　　冬凌草 R abdos ia r ubescens Hemsl . 为唇 1 . 1 　仪器 　CS - 930 1 双波长薄层扫描仪 日