产共轭亚油酸乳酸菌的筛选及产物分析.pdf

中国农业大学学报　2004, 9 (3):5 ～8 Journal o China Agricultural University 产共轭亚油酸乳酸菌的筛选及产物分析 1, 2 1 1 1 1 1 张中义 　胡锦蓉 　刘 萍 　苗士达 　柴秋儿 　孙君 (1.中国农业大学食品科学与营养工程学院, 北京100083; 2.郑州轻工业学院食品科学与生物工程系, 郑州 4 50002) 摘　要　为获得共轭亚油酸(CLA)乳酸菌的菌种, 从酸菜汁中筛选出1 株CLA 生成能力较强的的乳酸菌, 发酵液 中CLA 生成量为 267.5 μg ·mL-1。此株为革兰氏阳性杆菌, 过氧化氢酶阴性,4 5 ℃下生长, 15 ℃基本不生长。基 于生化试验和培养基成分的利用情况, 鉴定为植物乳杆菌Lactobacillus p lan tarum 。经气相色谱检测, 该菌种合成 的CLA 产物为 c9,t 11/t9, c 11-CLA 和 t 10, c 12-CLA 的混合物。 关键词　共轭亚油酸;乳酸菌;植物乳杆菌 中图分类号　Q 939.117 　　　　文章编号　1007-4333 (2004)03-0005-04　　　　文献标识码　A Screening of conjugated linoleic acid producing lactic acid bacteria and its product analysis 1,2 1 1 1 1 1 Zhang Zhongyi , HuJinrong , Liu Ping , Miao Shida , Chai Qiuer , SunJunshe (1.College of Food Scienceand Nutritional Engineering, China gricultural University, Beijing 100083, China; 2.Department of Food and Biology Engineering, Zhengzhou Instituteof Light Industry, Zhengzhou450002, China) bstract　The st udy was carried out t o obt ain conj ugat ed linol eic acid (CLA)producing lactic acid bact eria.A lact ic acid bact eria L was isol at ed f rom pi ckle juice samples w hich have t he higher v alue of CLA forming ability, it s CLA pro- 18 duction in t he supernat ant was 267.5 μg ·mL-1.The cult ure was found to be Gram posit ive, rods and cat al ase nega- tiv e.It grew at 45 ℃but not at 15 ℃, ident ified as Lact obacillus plant arum, on the basis of the biochemical charact er- istics and t he ut ilization of s ubstrat es.The production of L18 cult ure is t he mixt ure of c9, t 11/t9, c11-CL