HPLC指纹图谱技术用于滋肾通关胶囊提取工艺评价研究.pdfVIP

HPLC 指纹图谱技术用于滋肾通关胶囊提取工艺评价研究Δ ＊ 周琴妹 ，张登山，陈晓斌（江苏省中医院，南京市 210029） 中图分类号 R283.65；R927 文献标识码 A 文章编号 1001-0408（2011）11-1005-03 摘 要 目的：建立滋肾通关胶囊的高效液相色谱（HPLC ）指纹图谱，用于评价其提取工艺的优劣。方法：色谱柱为Kromasil C18 （250mm×4.6mm ，5μm ），流动相为乙腈-33mmol ·L-1磷酸二氢钾缓冲液（梯度洗脱），检测波长为317nm ，柱温为25 ℃，流速为1 mL ·min-1，进样量为10μL 。用盐酸小檗碱、芒果苷、桂皮醛3种对照品为参照物，建立混合药材的指纹图谱，并以此为标准图谱指 认了供试品中对应的成分。结果：在建立的色谱条件下，样品中的成分得到了较好的分离。以70％的乙醇为提取溶剂优于其他2 种溶剂。结论：在滋肾通关胶囊的提取工艺中，指纹图谱的多成分评价方法优于常规的单一指标评价方法。 关键词 高效液相色谱法；指纹图谱；参照峰；提取工艺；提取液；滋肾通关胶囊 Application of HPLC Fingerprint in Evaluation of Extraction Process of Zishen Tongguan Capsules ZHOU Qin-mei ，ZHANG Deng-shan ，CHEN Xiao-bin （Jiangsu Provincial Hospital of Traditional Chinese Medi- cine ，Nanjing 210029，China ） ABSTRACT OBJECTIVE ：To establish the HPLC fingerprint of Zishen tongguan capsule and evaluate the pros and cons of ex- traction process. METHODS ：The analytical column was Kromasil C18（250mm×4.6mm ，5 μm ）column with acetonitrile-33mmol · L-1 monopotassium phosphate buffer solution （gradient elution ）as mobile phase at flow rate of 1mL ·min-1. The detection wave- length was set at 317 nm and the column temperature was 25 ℃. The injection volume was 10 μL using berberine hydrochloride ， mangiferin and cinnamaldehyde as control. The HPLC fingerprint of mixed medicine was established and uses as standard chromato- grams. The corresponding composition of samples had been identified. RESULTS ：Chemical compositions of samples had been sepa- rated successfully by established method. 70％ ethanol was better than other two solvent for extracting. CONCLUSION ：The multi-component evaluation method of HPLC fingerprint is better than the normal single-component evaluation method in the study of extraction process of Zishen tongguan capsule. KEY WORDS HPLC ；Fingerprint ；Reference chromatographic peak ；Extraction process ；Extracts ；Zishen tongguan capsules 在中药提取制备工艺