蛋黄中胆固醇含量测定方法的研究.pdfVIP

检测分析 蛋黄中胆固醇含量测定方法的研究 张 旭，胡 艳，蒋桂韬，王向荣，李昊帮，戴求仲* （湖南省畜牧兽医研究所，长沙 410131 ） 摘 要：比较研究了高效液相色谱法（HPLC 法）、皂化- 比色法和试剂盒法3 种测定蛋黄胆固醇含量的方法， 结果表明，HPLC 法能够准确、快速的分析出蛋黄中的胆固醇含量，较皂化- 比色法和试剂盒法的准确度高。 HPLC 法测定蛋黄胆固醇含量的前处理为：用95%乙醇处理蛋黄稀释液，再用乙醚和石油醚混合萃取出胆固醇， 用氮气吹干后加无水乙醇溶解，经0.45 μm 有机微孔滤膜过滤后制成样品。色谱条件为：C18 柱，乙腈-异丙醇 （4 1，V/V ）作为流动相，流速为0.8 mL ·min-1 ，柱温35 ℃，检测波长210 nm ，进样量10 μL ，出峰时间14.578 min 。采用外标法定量。 关键词：蛋黄；胆固醇；HPLC ；比色；试剂盒 + 中图分类号：S879.3；S851.34 7 文献标志码：A 文章编号：1001-0084（2011）12-0035-04 Study on Determination Method of Egg Yolk Cholesterol Content ZHANG Xu, HU Yan, JIANG Guitao, WANG Xiangrong, LI Haobang, DAI Qiuzhong* （Hunan Institute of Animal Science and Veterinary Medicine, Changsha 410131, China） Abstract: The essay contrasted and studied on three methods of determinating the egg yolk cholesterol con⁃ tent. The three methods were High performance liquid chromatography(HPLC), saponification-colorimetric method, and kit method. The results showed that HPLC method can accurately and fast analysis cholesterol content in egg yolk. The accuracy of HPLC was better than saponification-colorimetric method and kit method. The pre-treatment methods was using 95% ethanol dispose yolk diluent, then using ether and petroleum ether mixed extract cholester⁃ ol, afterwards blow dry with nitrogen and add anhydrous alcohol to made dissolve, filtrate with 0.45 μm organic mi⁃ croporous membrane filter, so the sample is made. Chromatographic conditions was using C18 column, acetoni⁃ trile-isopropanol(4 1,v/v) as mobile phase, flow velocity was 0.8 mL ·min-1, column temperature was 35 ℃, detected wavelength was 210 nm, sample amount