抗疲劳活性研究.pdfVIP

Carbohydrate Polymers 74 (2008) 868–874 Contents lists available at ScienceDirect Carbohydrate Polymers journal homepage: www.elsevi /locate/carbpol Morphological and structural characterization of a polysaccharide from Gynostemma pentaphyllum Makino and its anti-exercise fatigue activity Ai-Ping Chi a,c,*, Jin-Ping Chen b, Zhe-Zhi Wang a, Zheng-Yi Xiong c, Qiu-Xia Li c a Key Laboratory Medicinal Plants and The Natural Resources of Medicinal Chemistry of Ministry of Education, 199, Changannan Road, Xi’an, Shaanxi 710062, China b The Department of Food Engineering, Shaanxi Normal University, Xi’an 710062, China c Institute of Exercise Biology, Shaanxi Normal University, Xi’an 710062, China a r t i c l e i n f o a b s t r a c t Article history: The crude polysaccharide was obtained from Gynostemma pentaphyllum Makino by water extraction fol- Received 15 March 2008 lowed by ethanol precipitation. The polysaccharide was successively purified by chromatography on Received in revised form 4 May 2008 DEAE-52 and SephadexG-150 column, and three polysaccharide fractions were obtained and termed Accepted 6 May 2008 GPP1-a, GPP2-b, and GPP3-a, respectively. The administration with GPP1-a markedly prolonged exhaus- Available online 16 May 2008 tive exercise time of the mice. Structural features of GPP1-a were investigated by a combination of instru- mental and chemical analyses, including atomic force microscope (AFM), scanning electron microscope Keywords: