白术多糖葡萄糖的含量测定_邱细敏.pdfVIP

( ) 2004, 1( 1) J of Hunan Normal Univ( Med S i) 47 X 邱细敏, 唐小异, 刘胜姿, 凌 晓, 戴丽华, 毛秀华 ( , 410006) [ ] : 研究和分析测定白术多糖葡萄糖的含 . : 采用水提醇沉法提取白术多糖, 用硫酸- 苯酚 法在489. 3 nm 处用紫外分光光度法测定, 用酶法在505 nm 处测定葡萄糖的含 . : 浙江白术与怀化白术多糖 含 差异较大, 测得结果平均回收率103. 1% , RSD 为1. 16% . : 简便, 快速, 准确, 可靠, 可用于不同产地白术 的鉴别和质 控制. [ ] 白术; 多糖; 葡萄糖; 分光光度法; 含 测定 [ ] R28411 [ ] A [ ] 1673-016x( 2004) 01-0047-03 Study on Content of Polysaccharide Dextrose in Atractylodes Macrocephala Koidz QI UXi-min , TAN G Xiao-yi , Liu Sheng-z i , LIN Xiao , et al ( Medi al College, Hunan Normal University, Changsha 410006, China) [ Abstract] Objective To study and determine the ontent of polysa haridedextrose for the dis- tin tion of atra tylodes ma ro ephala koidz. Methods The polysa haride was extra ted by water distillation and was deposited by al ohol. The ontent of polysa haride was determined by UV spe trophotometry at the wavelength of 489. 3 nm and by enzymati method at the wavelength of 505 nm. Results There are great dis- tin t between ontent of polysa haride of atra tylodes ma ro ephala koidz in Zhejiang and huaihua, the average re overy was 103. 1% , with RSD of 1. 16% . Conclusion The method UV spe trophotometry an be used to distinguish atra tylodes ma ro ephala koidz ( from different pla es) and to be its simple rapid a urate quality ontrol method. [ Key words] Atra tylodes Ma ro ephala Koidz; polysa haride; dextrose; spe trophotometry; de