肺炎克雷伯菌KPC-2酶的临床调查.pdfVIP

医学论坛 生物技术 世界 肺炎克雷伯菌KPC-2酶的临床调查 杨春艳 (铁岭卫生职业学院 辽宁铁岭 112000) 摘要: 目的:了解从中国医科大学附属第一医院临床患者分离的肺炎克雷伯菌产生KPC酶的情况。方法:筛选亚胺培南或(和)美洛培南的最小抑菌 浓度2~4 μg ／mL或(和)抑菌环直径16~21mm肺炎克雷伯菌,采用CLSI推荐的改良Hodge试验进行碳青酶烯类抗菌药物耐药的表型检测,采用聚合酶链 反应(PCR)进行KPC-2的基因型检测。结果:从临床分离出的18株肺炎克雷伯菌中有2株Hodge试验阳性,但KPC-2的PCR结果阴性。结论:医大一院临 床已经存在对碳青霉烯酶类抗生素耐药的肺炎克雷伯菌,但具体是哪一类酶尚不清楚,有待进一步研究。 关键词:肺炎克雷伯菌 碳青霉烯酶 KPC酶 改良的Hodge试验 PCR 中图分类号:R37 文献标识码:A 文章编号:1674-2060(20 15)04-0068-02 Abstract: Objective:In order to understand the situation the KPC enzyme arising from Klebsiella pneumonia isolated by the patients in the First Affiliated Hospital of China Medical University. Method:Screen the Klebsiella pneumoniae by the minimum inhibitory concentration with 2 ~ 4 μg / mL with treatment by imipenem or (and) meropenem, or (and) the inhibition zone diameter of 16 ~ 21mm. the modified Hodge test recommended by CLSI was used to carbapenem antibiotic drug resistance ,the KPC-2 genotype was tested by PCR,Result:18 Klebsiella pneumoniae was isolated from the sample, two was positive by Hodge test, but the result of KPC-2 was negative by PCR.Conclusion:Klebsiella pneumoniae with carbapenem antibiotic resistance has been the existence in the First Affiliated Hospital of China Medical University, but it is unclear what specific enzyme, pending further study. Key Words: Klebsiella pneumoniae;Klebsiella pneumoniae carbapenemase;KPC Hodge test;PCR 肺炎克雷伯菌是医院感染常见的病原菌 ,此类菌易产超广谱 霉烯类药物的碳青霉烯酶;(3)青霉素结合蛋白(PBP)的改变;(4)主动 β-内酰胺酶(ESBLs),也有携带质粒介导的头孢菌素酶(Ampc酶)的 外排系统的活跃。KPC酶是目前引起肺炎克雷伯菌耐药的主要原 [1]