CTAB法提取火龙果基因组DNA的试验研究.pdfVIP

, Journal of Anhui Agri. Sci. 2008, 36( 13) : 5325- 5326 庆 马君叶 CTABDNA 1 1 1 2 2 韦 茜 , 蔡永强 , 金吉芬, 陶 刚 , 朱 英 ( 1. , 550100; 2. , 550006) 以火龙果嫩茎为材料, 采用CTAB 法对火龙果材料的基因组DNA 的提取 法进行试验, 经采用调整试验顺序适当延长有机溶剂 的抽提时间和增加抽提次数提高提取DNA 的得率和纯度, 有效地克服了提取过程中粘液物质的干扰结果表明, 该 法可普遍适用 于仙人掌科量天尺属植物的DNA 提取, 所得DNA 纯度和得率能满足进一步研究的要求 火龙果; 基因组; DNA; 提取 Q 819 A 0517- 6611(2008) 13- 05325- 02 Experimental Study on the Extraction of GenomicDNAfromHy locereus undutus by CTAB method WEI Qian et al ( Guizhou Ins i u e of Frui Tree , Luodian, Guizhou 550100) Abstract Wi h ender s ems of Hylocereus undutus as ma erials, he experimen on he ex rac ion me hod of genomic DNA from ma erials H . undutus w as made by using CATB ex rac ion. Through adjus ing he experimen order, prolonging he ex rac ion ime of organic solven properly, increasing he ex- rac ion imes, enhancing he yield and puri y of he ex rac ed DNA, he dis urbance of mucous subs ance in he ex rac ion process was effec ively over- come. The resul s showed ha his me hod was generally sui able f or DNA ex rac ion from H . undatus plan s of Cac aceae and he puriy and yield of he ob ained DNA could mee he demands of fur her research. ey words Hylocereus undutus; Genome; DNA; Ex rac ion ( Pi aya) (Hylocereus undatus) , 1. 5 ml º ( Seleniereus ) , 600 Ll 65 e ( CTAB ) , 65 e , , , , 1 h, 5~ 10 min 1 »1 , 4 e ( PBCl 1B1) , 10 min, , 12 000 r/ min 20 min¼ 1. 5 ml , , , 0. 1 4 e KAc, 1- 20 e , , , - 20 e 20 min DNA ½DNA 1. 5 ml , , 70% 2 , ¾ , , 4 e T E , 4 e 2 h DNA ¿ DNA »~ ½1, 4 e TE 4 e 1 [ 1] 1. 1 ( ) , - 20 1. 4. 2 ¹DNA : DNA ( 100~ e