人脐带间充质干细胞分离培养及向脂肪与成骨细胞的分化.pdf

中同组织丁程研究与临床康复第。，4誊．筹74期2010—04—02出版 JournalofClinicalRehabi|itat^，eTissue Research VoL Engineering Apn|2．201014．No．14 人脐带间充质干细胞分离培养及向脂肪与成骨细胞的分化水水★△o 何绍清’～，罗振宇1～，刘秋英1～，周向荣△2～，邓铭权△2～，罗新4，姚润斯4，高志5，王一飞O’‘2 Isolationofhumanumbilicalcord stemcellsdifferentiationinto and adipocytes mesenchymal andosteblasts ’Biomedicine Researchand ACKGROUND：Culturemethodand in humanumbilicaIcord—denved condition．isolation aredifferent efficiency reported Centre Development stem esenchylllalcells．whichlackofunifiedidentificationstandards．Therefore．itis toestablisha and ofJinan necessary high·efficiency University, DnomicaIculture forhumanumbiliceIcord．derived stem Guangzhou systam mesenchymalcalls(hUCMSCs)． BJECTIVE：To 510632。Guangdong isolatehUCMSCsandinduceddifferentiateinto andosteblasts． adipocytes Province，China； ETHODS：ThehUCMSCswereisolated觚humanumbilicalcordtissueadherenceand with by digestedcollagenase．The ‘Guangzhou and cells thencellswereinducedto ofthe were orphology，proliferationimmunophenotype3”passage analyzed。and osteogenic (Jinan)-HongKong