模拟移动床色谱及其在茶叶功能成分分离中的应用前景.pdfVIP

296 2011, Vol. 32, No. 15 食品科学 ※专题论述 模拟移动床色谱及其在茶叶功能成分分离中的 应用前景 1,2 1 1, 1 黄永东 ，江用文 ，江和源 * ，王 斌 ( 1.中国农业科学院茶叶研究所，农业部茶及饮料植物产品加工与质量控制重点开放实验室，浙江 杭州 3 10008 ； 2.广东省农业科学院农产品质量安全与标准研究中心，广东广州 5 10640) 摘 要：模拟移动床色谱是20 世纪60 年代发展起来的能实现连续分离的一种制备色谱，具有提高产品纯度和回收 率，提高分离效率和降低成本等优点。该技术已经在石油化工和食品医药等领域有重要应用，但是尚未被应用到 茶叶功能成分的分离。目前，有研究表明模拟移动床色谱能用于分离儿茶素。本文综述模拟移动床色谱的分离原 理和国内外的应用进展，并分析该方法在茶叶功能成分分离中的应用前景。模拟移动床色谱将成为儿茶素单体和茶 黄素单体制备研究的主要方向。 关键词：模拟移动床色谱；茶叶功能成分；儿茶素 Simulated Moving Bed Chromatography and Its Application Prospects in the Separation of Functional Components in Tea 1,2 1 1, 1 HUAN G Yong-dong ，JIAN G Yong-wen ，JIAN G He-yuan * ，WANG Bin (1. Key Laboratory of Tea and Beverage Crop Product Processing and Quality Control, Ministry of Agriculture, Tea Research Institute, Chinese Academy of Agricultural Sciences, Hangzhou 3 10008, China；2. Center for Quality Safety and Standard Research of Agricultural Products, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, China) Abstract: Simulated moving bed chromatography, developed in the 1960s, is a kind of continuous preparative chromatography with multiple advantages, such as improving product purity and recovery rate, increasing separation efficiency and reducing costs. This technology has been extensively used in the petrochemical, food and pharmaceutical industries, but has not yet been applied to separate functional components of tea. Current studies have demonstrated the successful separation of catechin by using simulated moving bed chromatography. In thi